Abstract: Objective The Objective of this study was to observe the effect of miR-182 targeted regulation of PI3K/AKT/FOXO3a signaling pathway on the biological behavior of glioma stem cells(GSCs),and its mechanism of action. Methods GSCs were successfully isolated from the lesion tissues of glioma patients,before they were cultured,passaged and identified.GSCs were divided into the blank control group(physiological saline solution),miR-182 mimics group(cells transfected with miR-182 mimics)and negative control group(cell transfected nonsense sequence).After cells transfected,the transfection efficiency of miR-182 mimics was observed under an inverted fluorescence microscope.The expression of miR-182 mRNA and biological behavior changes such as proliferation,migration,invasion and apoptosis in each group were detected.The expression of PI3K/AKT/FOXO3a signaling pathway related proteins(PI3K,p-AKT,and FOXO3a)were also detected. Results The expression of miR-182 mRNA in the miR-182 mimics group was significantly higher than that of the blank control and negative control groups(P＜0.01).There was no significant difference between the blank control and negative control groups(P＞0.05).The cell proliferation rate,migration and invasion abilities of miR-182 mimics group at 24,48 and 72 h after transfection were significantly higher than those of blank control and negative control groups(P＜0.05).The apoptosis rate was significantly lower than that of blank control and negative control groups after transfection for 24 h,48 h and 72 h(P＜0.01).There was no statistical difference between the blank control and negative control groups(P＞0.05).After transfected with miR-182 mimics,the expression of PI3K and p-AKT protein in GSCs were significantly higher than those of blank control and negative control groups(P＜0.01),and the expression of FOXO3a protein was significantly lower than that of blank control and negative control groups(P＜0.01).The expression of PI3K,p-AKT,and FOXO3a protein was not statistical difference between the blank control and negative control groups(P＞0.05). Conclusion miR-182 may enhance the proliferation,migration and invasion of GSCs and inhibit cell apoptosis by activating the PI3K/AKT/FOXO3a signaling pathway.It may be a new target for clinical treatment of glioma.

Key words: Glioma;Glioma stem cells;Microribonucleic acid;Phosphatidylinositol-3-kinase;Protein kinase B;Human forkhead box O protein 3a