TMIGD1在结肠癌中的表达及对结肠癌细胞增殖和侵袭的影响

doi:10.11904/j.issn.1002-3070.2021.03.008

Abstract

Abstract: Objective The aims of this study were to detect the expression of TMIGD1 in colon cancer tissues and its corresponding adjacent colonic epithelial tissues,the effect of overexpression of TMIGD1 on the biological behaviors of colon cancer cells and to explore the potential molecular mechanisms.Methods The bioinformatics database and immunohistochemical staining were used to detect the expression of TMIGD1 in colon cancer and its corresponding adjacent colon tissues,and analyze its relationship with different clinicopathological factors.The colon cancer SW480 cells were stably overexpressed TMIGD1 through the lentiviral packaging system.MTT,clone formation experiment,cell cycle kit and Western blot were used to detect the effect of TMIGD1 on proliferation,clone formation,cell cycle and the expression of key proteins in the cell cycle of colon cancer cells.The Western blot was used to detect the expression of EMT-related proteins in SW480 cells,and Transwell experiment was used to detect cell migration and invasion.Results The results of bioinformatics analysis and immunohistochemical staining showed that TMIGD1 was low expressed in colon cancer tissues(P＜0.05).The negative expression of TMIGD1 was closely related to the lymph node metastasis and TNM staging of colon cancer(P＜0.05).The results of MTT,clone formation experiments and cell cycle analyses showed that overexpression of TMIGD1 could inhibit the proliferation and colony formation,and increase the number of cell cycle at the G0/G1 phase,and inhibit the expression of Cyclin D1,while promoting the expression of p21 in colon cancer SW480 cells(P＜0.05).TMIGD1 had no effect on EMT protein,but after stably overexpressing TMIGD1,the migration and invasion abilities of colon cancer cells were significantly reduced,while the adhesion ability was significantly enhanced(P＜0.05).Conclusion TMIGD1 is low expressed in colon cancer.Overexpression of TMIGD1 can inhibit the proliferation,migration and invasion of colon cancer cells.

Key words: Colon cancer, TMIGD1, Cell proliferation, Cell invasion

CLC Number:

R735.3

WU Yunhua, TAN Min, WANG Zijun, SUN Xuejun, LI Fanni. The expression of TMIGD1 in colon cancer and its effect on the proliferation and invasion of colon cancer cells[J]. Journal of Practical Oncology, 2021, 35(3): 237-242.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2021.03.008

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2021/V35/I3/237

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The expression of TMIGD1 in colon cancer and its effect on the proliferation and invasion of colon cancer cells

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