Journal of Practical Oncology ›› 2023, Vol. 37 ›› Issue (1): 32-38.doi: 10.11904/j.issn.1002-3070.2023.01.006

• Basic Research • Previous Articles     Next Articles

Effects of miR-765 targeting ARID1A on the invasion and migration of colorectal cancer cells

ZHOU Junfeng1, ZENG Zhiyao1, LUO Yongfu1, HE Chao2   

  1. 1. Department of Gastrointestinal Surgery,Yongzhou Central Hospital,Yongzhou 425000,China;
    2. Emergency Department,The First Affiliated Hospital of South China University
  • Received:2022-02-25 Revised:2022-12-08 Online:2023-02-28 Published:2023-03-21

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Abstract: Objective The aim of this study was to investigate the effect of microRNA-765(miR-765)targeting AT-rich interactive domain-containing protein 1A(ARID1A)on the invasion and migration of colorectal cancer cells. Methods Colorectal cancer tissues and adjacent tissues were collected from 35 patients with colorectal cancer,SW480 cells were cultured and divided into the control group,siRNA NC group,miR-765 siRNA group,mimic NC group,and miR-765 mimic group.qRT-PCR was used to detect the expression of miR-765 and ARID1A mRNA.Transwell assay was used to detect the migration and invasion of SW480 cells.Western blot was used to detect the expression of ARID1A,matrix metalloproteinase-9(MMP-9)and vascular endothelial growth factor(VEGF)protein.Dual luciferase assay was used to detect the targeting relationship between miR-765 and ARID1A. Results Compared with adjacent tissues,the level of miR-765 in colorectal cancer tissues was significantly increased,and the ARID1A at levels of mRNA and protein were significantly reduced(P＜0.001),and there was a significant negative correlation between miR-765 and ARID1A mRNA in colorectal cancer tissues(r=-0.768,P＜0.001).Compared with TNM stage Ⅰ-Ⅱ patients,the level of miR-765 in colorectal cancer tissues of patients with TNM stage III to IV was significantly increased(P＜0.01),and the expression of ARID1A protein was significantly decreased(P＜0.001).After knockdown -765 expression,the number of migration and invasion,miR-765 level,the levels of MMP-9 and VEGF protein were significantly reduced in SW480 cells,and the expression of ARID1A protein was significantly increased(P＜0.05).After overexpression of miR-765,the number migration and invasion,miR-765 level,the levels of MMP-9 and VEGF protein were significantly increased in SW480 cells,while the expression of ARID1A was significantly reduced(P＜0.05).miR-765 targeted and regulated ARID1A expression. Conclusion miR-765 can promote the invasion and migration of SW480 cells by targeting and inhibiting the expression of ARID1A.

Key words: microRNA-765, AT-rich interactive domain-containing protein 1A, Colorectal cancer, Migration, Invasion

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