lncRNA AP000487.1在食管癌中的作用及机制研究

doi:10.11904/j.issn.1002-3070.2023.02.006

Abstract

Abstract: Objective To explore the role and mechanism of survival-related lncRNA AP000487.1 in the occurrence and development of esophageal cancer. Methods qRT-PCR was used to verify the expression of lncRNA AP000696.1,LINC00689,LINC00900 and AP000487.1 that significantly associated with overall survival of esophageal cancer patients in esophageal cancer tissues and cell lines.Cell proliferation was detected by CCK-8 assay,cell invasion was evaluated by Transwell invasion assay,and cell migration was detected by scratch assay.The expression of TLR/NF-κB signaling pathway related proteins was detected by Western blot and qRT-PCR. Results The expression of AP000487.1 was significantly up-regulated in ECA109 cells,TE-1 cells,and ESCA tissues(P＜0.001).Silencing AP000487.1 in ECA109 cells and TE-1 cells significantly inhibited the proliferation,invasion and migration of ESCA cells.Overexpression of AP000487.1 significantly enhanced the proliferation,invasion and migration.Western blot was used to detect pathway-related proteins,and the results showed that silencing AP000487.1 could inhibit the TLR/NF-κB signaling pathway and down-regulate the expression of p-p65 and p-IκB in TE-1 cells;Compared with the Smart silencer-NC group,the expressions of TNF-α(P＜0.001),IL-1β(P＜0.01)and IL-6(P＜0.01)in the Smart silencer-AP000487.1 group were significantly down-regulated. Conclusion AP000487.1 is highly expressed in esophageal cancer cell lines and tissues,and its abnormal expression promotes the proliferation,invasion and migration of ESCA by regulating the TLR/NF-κB signaling pathway.AP000487.1 may be a potential therapeutic target for ESCA.

Key words: Esophagus cancer, lncRNA AP000487.1, Toll-like receptor, NF-κB

CLC Number:

R735.1

LIU Jing, CHU Yanjie, PEI Fenghua, XU Ruiling, WANG Yanbo. Role and mechanism of lncRNA AP000487.1 in esophageal cancer[J]. Journal of Practical Oncology, 2023, 37(2): 123-129.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2023.02.006

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2023/V37/I2/123

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Role and mechanism of lncRNA AP000487.1 in esophageal cancer

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