长链非编码RNA OTUD6B-AS1对肺腺癌A549细胞增殖、侵袭和迁移能力的影响

doi:10.11904/j.issn.1002-3070.2024.01.003

Abstract

Abstract: Objective The aim of this study was to investigate the effects of LncRNA OTUD6B-AS1 on the proliferation,migration and invasion of lung adenocarcinoma A549 cells. Methods Lung adenocarcinoma A549 cell line was cultured in vitro,and transient transfection of OTUD6B-AS1 and empty plasmid group were used as the control group.Overexpression and control cell models were constructed,and divided into OTUD6B-AS1 overexpression group and empty plasmid group(NC group).The cell model was divided into the empty plasmid group(NC group)and OTUD6B-AS1 overexpression group.The transfection efficiency of OTUD6B-AS1 mRNA was verified through qRT-PCR.The CCK-8 experiment was used to detect the effect of OTUD6B-AS1 on the proliferation activity of lung adenocarcinoma cells,and the Transwell assay was used to detect the effect of OTUD6B-AS1 on the migration and invasion ability of lung adenocarcinoma cells. Results Compared to the NC group,the overexpression OTUD6B-AS1 group had a significant increase in the expression of OTUD6B-AS1(P＜0.05).The CCK-8 assay results showed that the proliferation activity of A549 cells in the OTUD6B-AS1 overexpression group was significantly reduced compared to the NC group(P＜0.05).The results of the Transwell assay showed that the OTUD6B-AS1 overexpression group had significantly lower cell migration and invasion abilities than the NC group(P＜0.05). Conclusion Overexpression lncRNA OTUD6B-AS1 in lung adenocarcinoma A549 cells can significantly inhibit the proliferation,migration,and invasion ability of A549 cells.

Key words: Long chain non coding RNA, Ovarian tumor domain containing 6B antisense RNA1, Lung cancer, Migration, Invasion

CLC Number:

R734.2

BO Yun, HANG Wenlu, ZUO Shurun, Dong Yuan, LI Haiquan. Effects of long non-coding RNA OTUD6B-AS1 on proliferation,invasion and migration of lung adenocarcinoma A549 cells[J]. Journal of Practical Oncology, 2024, 38(1): 13-17.

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http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2024/V38/I1/13

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Effects of long non-coding RNA OTUD6B-AS1 on proliferation,invasion and migration of lung adenocarcinoma A549 cells

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