Objective To investigate the clinical factors on postoperative radiation induced lung injury in patients with breast cancer,in order to optimize the radiotherapy plan.Method 96 patients with breast cancer who had received postoperative radiotherapy were analyzed,the relationship between the incidence of radiation injury and radiation field,radiation dose,chemotherapy drugs,age,underlying disease of the lung were observed.Results 19 patients developed radiation induced lung injury.The incidence of radiation induced lung injury were related with radiation field,radiation dose(P＜0.05).Conclusion The design of radiation field and increasing radiation dose are the major factors to increasing incidence of radiation induced lung injury in patients with breast cancer.

Objective To explore the effect SiRNA mediated silencing of cFLIP gene combined with Epirubicin on apoptosis of MCF-7 cells.Methods Chemosynthesis double strand siRNA of c-FLIP,transfer to MCF-7 cell,then detect the expression of c-FLIP and Caspase-8 protein using Western blot;detect the apoptosis with flow cytometry when c-FLIP-siRNA interference or combined with Epirubicin.Results c-FLIP-siRNA can inhibit c-FLIP protein expression effectively(P＜0.05),promote cells apoptosis(P＜0.05).Combined using of c-FLIP-siRNA and Epirubicin can promote MCF-7 cell apoptosis more significantly,compared with Epirubicin using alone(P＜0.05).Conclusion c-FLIP-siRNA can promote MCF-7 cell apoptosis,and increase the anti-tumor effect of Epirubicin.

Objective To investigate the growth properties of human breast cancer cell line MCF7-pAcGFP labelled with green fluorescent protein.Methods Human breast cancer cell lines MCF-7 were transfected with pAcGFP-C1 by Fugene HD Transfection Reagent.The cells which express the pAcGFP gene were selected by geneticin(G418).The growth curve were detected by MTT,the cell cycle were detected by flow cytometry,and the expressions of 11 proteins in MCF7-pAcGFP cells were detected by western blot.Results The growth curve and cell cycle of MCF7-pAcGFP cells were similar to untransfected ones(P＞0.05).There were no significant diffrence in the expression of Cyclin D1,p27,Fas,FasL,Bcl-2,Bax,Akt,p-Akt(Ser473),p-Akt(Thr308),ERK and p-ERK between the MCF7-pAcGFP cells and MCF-7 cells.Conclusion Compared with parents cells,there was no significant change in growth properties of MCF7-pAcGFP cells.So MCF7-pAcGFP cells may provide a basis for construction a ideal animal model to research molecular mechanisms of growth in human breast cancer.

Objective To investigate the feasibility of laparoscopic resection of colorectal cancer.Methods Retrospective analysis of our hospital from January 2009 to December 2009 of the implementation of laparoscopic resection in 97 colorectal cancer patients with clinical data.Results Dixon laparoscopic surgery 32 cases,Miles operation in 28 cases,Hartmann 5 patients,sigmoid colon cancer radical operation in 16 cases,left colon cancer radical operation 12 cases,right colon cancer radical operation 4 cases.97 cases of colorectal cancer patients with lymph node dissection up to the third station.Operative time was 193.1±58.3min.Surgical bleeding 58.3±19.6mL,postoperative gastrointestinal function recovery time 2.1±0.7d,an average of 53h.Postoperative hospitalization days 15.1± 3.1d.No cancer was found in two cutting edges.The number of lymph node dissection 8.6±4.9 pieces.Patients were followed up so far,in the 97 cases of postoperative,one case of liver metastasis,one died of postoperative myocardial infarction in two months later.No local,abdominal lymph node metastasis and recurrence,and incision metastasis.Conclusion Laparoscopic resection of colorectal cancer with a minimally invasive,safe,small incision,clear vision and rapid recovery features,is feasible.

Objective To investigate the effects of CCR7 activation in vitro proliferation,chemotaxis and invasion of human colon cancer SW620 cells.Methods SW620 proliferation ability was examined by MTT assay and plate clone formation assay.Chemotaxis and invasion of SW620 cells were investigated using Boyden chamber assay with the absence and presence of CCL21.Results Compared with those in the control group,the numbers of the proliferative cells,forming plate cells clones and cells permeating the septum of the Boyden chamber were significantly increased in the CCL21 group(P＜0.01).Conclusions CCR7 activation could promote proliferation,chemotaxis and invasion of SW620 cells,and it maybe play a role in lymph node metastasis of colon cancer.To further study CCR7 may help to elucidate the mechanism of lymph node metastasis in human colon cancer.

Objective To design siRNA targeting to survivin and construct an eukaryocytic expression vector pSUPER/survivin,then transfected it into cervical cancer cell line HeLa and evaluated the effects of siRNA against survivin gene on cell cycle of cervical cancer cell line HeLa irradiated with γ-rays.Methods The primer was synthesized,siRNA targeting to survivin was obtained,linked with the vector pSUPER and sequenced.The correct pSUPER/survivin was transfected into HeLa cells and the expression of survivin gene in Hela cells was detected by RT-PCR and Flow Cytometry(FCM).In the present study,the effects of survivin gene transfection combined with irradiation on cell cycle distribution were analysed by FCM.Result Recombinant eukaryocytic expression vector pSUPER/survivin was successfully constructed.FCM results indicated that the expression of survivin gene is significantly suppressed in pSUPER/survivin transfected cells compared with non-transfected cells or empty vector pSUPER transfected cells.After γ-ray irradiation,HeLa cells transfected with pSUPER/survivin induced less proportion of S phase and G₂/M arrest.Conclusion Recombinant eukaryocytic expression vector pSUPER/survivin was successfully constructed.siRNA against survivin enhanced radiosensitivity through execution of apoptosis,induction less proportion of S phase and G₂/M arrest,it may be a potential target for improving radiosensitivity

Objective To investigate the expression of RET and VEGF-C in papillary carcinoma of thyroid,Soasto the role and correlation of RET and VEGF-C.Methods Detecting the expression of RET and VEGF-C in 60 papillary carcinoma of thyroid tissues and 10 normal thyroid tissues by immunohistoche mical Envision two-step method,analyzing the correlation between the protein expression rate and clinical and pathological characters,and also the correlation between the expression of RET and VEGF-C.Results Expression of every oncogene was significantly different between papillary carcinoma of thyroid tissues and normal thyroid tissues(P＜0.05).The positive expression rate of RET and VEGF-C were significantly associated with cervical lymph node metastasis and clinical stages(P＜0.05),but not associated with sex,age,primary site of the tumor(P＞0.05).The positive expression of RET closely related to the positive expression of VEGF-C(P＜0.05).Conclusion The results demonstrate that RET may play an important role in the cervical lymph node metastasis of papillary carcinoma of thyroid,the expression of VEGF-C.RET and VEGF-C should be used as an important marker of cervical lymph node metastasis.

Objective To explore the role of RUNX3 and OPN in pathogenesis,development,invasion,metastasis of esophageal carcinoma and the correlation between the expression of RUNX3 protein and OPN protein.Methods Immunohistochemisty was used to detect the expression of RUNX3 and OPN in normal esophageal tissues,the tissues of atypical hyperplasia of tumor-adjacent and esophageal carcinoma tissues collected from 60 esophageal carcinoma specimens.Results RUNX3 expression density is different in normal esophageal tissues,the tissues of atypical hyperplasia of tumor-adjacent and esophageal carcinoma tissues(78.33%、43.48%、31.67%).Expression of RUNX3 protein is correlated with the infiltrative depth and tumor TNM stage of esophageal squamous cell carcinoma(P＜0.05).OPN expression density is different in normal esophageal tissues,the tissues of atypical hyperplasia of tumor-adjacent and esophageal carcinoma tissues(0%、34.78%、76.67%).Expression of OPN protein is correlated with the infiltrative depth,lymph node metastasis and tumor TNM stage of esophageal squamous cell carcinoma(P＜0.05).Conclusion The detection of the RUNX3 expression combined with OPN expression might have more significant meaning to explore the mechanism of pathogenesis development,invasion and metastasis of esophageal carcinoma and to predict the prognosis of esophageal carcinoma.

Objective To investigate the expression of CD34 and MMP-9 in osteosarcoma,as well to explore their relationship and clinical significance.Methods CD34 and MMP-9 were detected by S-P immunohistochemical method in 48 cases of paraffin-embedded sections of osteosarcoma.Results The positive rate of CD34 and MMP-9 in human osteosarcoma is 62.50% and 56.25% respectively,significantly higher than that of osteochondroma.The expression of CD34 is significantly associated with the histological grades and lung metastasis(P=0.014,P=0.002).The expression of MMP-9 is significantly correlated with histological grades and lung metastasis(P=0.013,P=0.013).There is a significant correlation between the expression of CD34 and MMP-9(r=0.005,P=0.006).Conditions such as age and sex have no relation with the expression of CD34 and MMP-9.Conclusion CD34 and MMP-9 participate in the carcinogenesis and development of osteosarcoma,and there is synergetic action between them.

Objectives Explore the best experiment condition for touchdown PCR and clone-based sequencing to detect the methylation status of hsa-mir-34b gene promoter.Methods After the bisulfite treatment of the DNA extracted from lung cancer cell line A549,the primer was designed to amplify the promoter region of mir-34b gene.General PCR,nest PCR and touchdown PCR were employed to amplify the target fragment with different DNA polymerase.Then direct sequencing and cloning-based sequencing methods were performed to detect the methylation status.Results Compared to general PCR,touchdown PCR and nest PCR was used alone,touchdown PCR together with nest PCR was much easier to amplification;HotStart Taq was the suitable DNA polymerase in the PCR reaction system.Compared to the direct sequencing,cloning-based sequencing could find the methylation status more clear.Conclusions HotStart Taq DNA polymerase,touchdown PCR together with nest PCR and cloning-based sequencing methods were recommend to be used to detect the methylation status after the DNA underwent the bisulfite treatment,by which we could obtain the methylation patterns clearly with high sensitivity.