Abstract: Objective The function of cIAP1 in the progression of ovarian cancer has not been clarified.This study is to explore the involvement of cIAP1 in regulating biological behaviors of ovarian cancer cells by using RNA interference(RNAi)technology.Methods The short hairpin RNA plasmid targeting cIAP1 was constructed and transfected into Skov3 cells.The levels of cIAP1 mRNA and protein were investigated by RT-PCR and Western Blot respectively.MTT assay and flow cytometry were used to evaluate cell proliferation and apoptosis.Results The rate of cIAP1 transfection was 74.7% performed by flow cytometric analysis.cIAP1 expression was significantly down-regulated at both mRNA and protein levels,which resulted in a decrease of cell proliferation and invasion capability in vitro.Conclusion This study implies that cIAP1 might play an important role in the progression of ovarian cancer,and it could be a potential target for therapeutic anti-cancer drugs.