Biglycan及FAK信号通路对结肠癌细胞侵袭、转移能力的影响及机制

doi:10.11904/j.issn.1002-3070.2015.05.013

实用肿瘤学杂志 ›› 2015, Vol. 29 ›› Issue (5): 444-449.doi: 10.11904/j.issn.1002-3070.2015.05.013

• 辽宁省肿瘤医院院庆专题 • 上一篇下一篇

Biglycan及FAK信号通路对结肠癌细胞侵袭、转移能力的影响及机制

谷小虎¹, 肖景东², 邢晓静¹

1.辽宁省肿瘤医院(沈阳 110042);
2.辽宁中医药大学

收稿日期:2015-06-26 出版日期:2015-10-28 发布日期:2015-10-30
通讯作者: 邢晓静,E-mail:13940066477@163.com
作者简介:谷小虎,男,(1973-),博士,主任医师,从事消化肿瘤的研究
基金资助:
辽宁省自然科学基金(201102111);国家自然科学基金项目(81201968)

Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells

GU Xiaohu¹, XIAO Jingdong², XING Xiaojing¹

1.Liaoning Cancer Hospital & Institute,Shenyang 110042,China;
2.Liaoning University of Traditional Chinese Medicine

Received:2015-06-26 Online:2015-10-28 Published:2015-10-30

摘要/Abstract

摘要： 目的观察Biglycan对局部黏着斑激酶(Focal adhesion kinase,FAK)活化的影响,探索其是否通过调控FAK信号通路影响结肠癌细胞的侵袭和迁移。方法构建过表达Biglycan的人结肠癌细胞系HCT116并施加FAK抑制剂处理。实验设置空白对照组(HCT116)、空载体对照组(Vector),空载体抑制剂处理组(Vector+PF-562271)、Biglycan过表达组(Biglycan)和Biglycan过表达抑制剂处理组(Biglycan+PF-562271)。抑制剂处理24h后,采用Western blot技术检测FAK、p-FAK在各组结肠癌细胞中的表达;采用Transwell实验分析各组结肠癌细胞的侵袭和迁移能力。结果 Biglycan过表达后会显著提高FAK的磷酸化水平,促进HCT116细胞的侵袭和迁移(P＜0.01);FAK抑制剂PF-562271能够明显降低p-FAK的表达,逆转Biglycan对结肠癌细胞侵袭和迁移能力有影响(P＜0.01)。结论 Biglycan通过激活FAK信号通路影响结肠癌细胞系HCT116的侵袭和转移。

关键词: Biglycan, 局部黏着斑激酶, 结肠癌, 侵袭, 迁移

Abstract: Objective To observe the influence of Biglycan on the focal adhesion kinase(FAK)activation and to explore whether it regulates the invasion and metastasis of colon cancer through FAK signaling pathway.Methods The overexpressive plasmid of Biglycan was constructed and then transfected into the colon cancer cell line HCT116.Meanwhile,FAK inhibitor was used to treat cells.Control group(HCT116),empty plasmid group(Vector),empty plasmid and inhibitor treatment group(Vector+PF-562271),Biglycan overexpression group(Biglycan),Biglycan overexpression and inhibitor treatment group(Biglycan+PF-562271)were set.Twenty four hours after exposure to inhibitor,the expression of FAK and p-FAK in each group was detected by Western Blot.The invasion and metastasis of colon cancer cells was detected by transwell assay.Results Overexpression of Biglycan significantly enhanced the phosphorylation level of FAK and promoted the invasion and metastasis of HCT116(P＜0.01).The inhibitor of FAK PF-562271 could significantly reduce the expression of p-FAK and reverse the effect of Biglycan on the invasion and metastasis of colon cancer cells.Conclusion Biglycan regulates the invasion and metastasis of colon cancer cells through activation FAK signaling pathway.

Key words: Biglycan, FAK, Colon cancer, Invasion, Metastasis

中图分类号:

R735.3

谷小虎, 肖景东, 邢晓静. Biglycan及FAK信号通路对结肠癌细胞侵袭、转移能力的影响及机制[J]. 实用肿瘤学杂志, 2015, 29(5): 444-449.

GU Xiaohu, XIAO Jingdong, XING Xiaojing. Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(5): 444-449.

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Biglycan及FAK信号通路对结肠癌细胞侵袭、转移能力的影响及机制

Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells

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