男乳癌与AR基因CAG重复多态性的相关性研究

doi:10.11904/j.issn.1002-3070.2015.06.003

实用肿瘤学杂志 ›› 2015, Vol. 29 ›› Issue (6): 492-496.doi: 10.11904/j.issn.1002-3070.2015.06.003

男乳癌与AR基因CAG重复多态性的相关性研究

崔佳琳¹, 黄睿², 姜永冬³, 韩继广³, 牛明³, 魏巍³, 郑伟³, 宋燕妮³

1.哈尔滨医科大学附属肿瘤医院检验科(哈尔滨 150081);
2.哈尔滨医科大学附属第二医院普外科;
3.哈尔滨医科大学附属肿瘤医院乳腺外科

收稿日期:2015-07-08 出版日期:2015-12-28 发布日期:2015-12-28
通讯作者: 宋燕妮,E-mail:yannimd@126.com E-mail:yannimd@126.com
作者简介:崔佳琳,女,(1981-),本科,检验师,从事临床医学检验的研究
基金资助:
黑龙江省卫生厅课题(2013066);黑龙江省自然基金课题资助(H2015050);国家自然基金课题资助(H81302286)

Related research of male breast cancer and CAG repeat polymorphism of AR gene

CUI Jialin¹, HUANG Rui², JIANG Yongdong³, HAN Jiguang³, NIU Ming³, WEI Wei³, ZHENG Wei³, SONG Yanni³

1.Clinical Laboratory,The Affiliated Tumor Hospital of Harbin Medical University,Harbin 150081,China;
2.Department of Surgery,The Second Affiliated Hospital of Harbin Medical University;
3.Department of Breast Surgery,The Affiliated Tumor Hospital of Harbin Medical University

Received:2015-07-08 Online:2015-12-28 Published:2015-12-28

摘要/Abstract

摘要： 目的探讨雄激素受体(AR)基因外显子CAG重复频数多态性与男性乳腺癌(男乳癌)发病风险的关系。方法研究对象为40例男性乳腺癌患者和40例男性健康者,从外周血提取DNA,对AR基因外显子CAG编码序列进行PCR扩增、测序和计算CAG重复频数,用χ²检验和Logistic回归分析AR基因CAG重复频数长度对男乳癌发病风险的影响。结果男性乳腺癌病例组和对照组CAG重复频数长度存在差异,其差异具有统计学意义,CAG重复频数长度超过22男性乳腺癌发病风险是重复频数长度少于21的3.52倍(OR=3.52,P=0.036)。结论 AR基因CAG重复频数长度是预测男性乳腺癌发病风险的指标,较长(超过22)的CAG重复序列可增加男乳癌的发病风险。

关键词: 男性乳腺癌, AR, CAG重复频数, 多态性

Abstract: Objective To investigate the correlation between(CAG)n repeat polymorphism of androgen receptor(AR)gene and male breast cancer.Methods 40 cases of male breast cancer and 40 controls were collected.DNA was extracted from peripheral blood and the AR gene CAG coding exon sequences for PCR amplification,sequencing and calculated the number of CAG repeats frquency.χ² test and Logistic regression analysis were used assess the AR gene CAG repeat length frequency affect the number of male breast cancer risk.Results There was statistically significant difference in male breast cancer cases and controls the number of CAG repeat length frequency.Man for whom the(CAG)n≥22 repeat sequence had 3.52 times risk of male breast compared(CAG)n≤22(OR=3.52,P=0.036).Conclusion AR gene CAG repeat length is a predictor of the frequency of male breast cancer risk.Longer CAG repeats can increase the risk of male breast cancer.

Key words: Male breast cancer, Androgen receptor, CAG repeat sequence, Polymorphic

中图分类号:

崔佳琳, 黄睿, 姜永冬, 韩继广, 牛明, 魏巍, 郑伟, 宋燕妮. 男乳癌与AR基因CAG重复多态性的相关性研究[J]. 实用肿瘤学杂志, 2015, 29(6): 492-496.

CUI Jialin, HUANG Rui, JIANG Yongdong, HAN Jiguang, NIU Ming, WEI Wei, ZHENG Wei, SONG Yanni. Related research of male breast cancer and CAG repeat polymorphism of AR gene[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(6): 492-496.

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男乳癌与AR基因CAG重复多态性的相关性研究

Related research of male breast cancer and CAG repeat polymorphism of AR gene

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