MicroRNA-27a对U251胶质瘤细胞的影响

doi:10.11904/j.issn.1002-3070.2016.04.011

摘要/Abstract

摘要： 目的探讨microRNA-27a(miR-27a)对U251胶质瘤细胞的影响。方法采用体外瞬时转染的方法过表达或抑制U251细胞中的miR-27a,MTT方法检测细胞的增殖情况,Transwell侵袭试验测定U251胶质瘤细胞的侵袭能力,Real-time PCR方法测定U251细胞中miR-27a和PPARγ的含量。结果抑制miR-27a可降低U251胶质瘤细胞增殖能力,减弱U251胶质瘤细胞侵袭能力,增加PPARγ的含量;过表达miR-27a促进U251胶质瘤细胞增殖能力,提高U251胶质瘤细胞侵袭能力,减少PPARγ的含量。结论抑制miR-27a有利于降低U251胶质瘤细胞的增殖能力和侵袭能力。

关键词: 微小RNA-27a, 胶质瘤, PPARγ, 增殖, 侵袭

Abstract: Objective To discuss the effect of microRNA-27a on U251 glioma cells.Methods Overexpression or inhibition of miR-27a in U251 glioma cells were done by transient transfection of miR-27a mimics or AMO-27a in vitro.Cell viability was detected by MTT assay.Invasion ability of U251 was detected by transwell invasion assay.The level of miR-27a and PPARγ were detected by real-time PCR.Results Under inhibition of miR-27a condition,the proliferation and invasiveness of U251 glioma cells were decreased.The level of PPARγ was significantly increased,whereas the level of miR-27a was decreased.Overexpression of miR-27a increased the proliferation and invasiveness of U251 glioma cells and decreased the level of PPARγ.Conclusion Inhibition of miR-27a is benefit for inhibiting the proliferation and invasiveness of U251 glioma cells.

Key words: MicroRNA-27a, Glioma, PPARγ, Proliferation, Invasiveness

中图分类号:

R739.41

刘国军,张俊,范永春,王庆锁,李伟. MicroRNA-27a对U251胶质瘤细胞的影响[J]. 实用肿瘤学杂志, 2016, 30(4): 346-350.

LIU Guojun,ZHANG Jun,FAN Yongchun,WANG Qingsuo,LI Wei. The effect of microRNA-27a on U251 glioma cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(4): 346-350.

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