RPL23-siRNA干扰片段的构建及筛选

doi:10.11904/j.issn.1002-3070.2016.06.002

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (6): 487-491.doi: 10.11904/j.issn.1002-3070.2016.06.002

RPL23-siRNA干扰片段的构建及筛选

彭文苗^1,2，张志敏¹，胡萌¹，余丽芳¹，章必成¹，饶智国¹，秦传蓉^1,2

1.中国人民解放军武汉总医院肿瘤科(武汉 430070)；
2.湖北中医药大学临床医学院

收稿日期:2016-07-15 出版日期:2016-12-31 发布日期:2016-12-27
通讯作者: 饶智国,E-mail:raozhiguo@hotmail.com
作者简介:彭文苗，男，(1990-)，硕士研究生，从事肿瘤疾病基础及临床的研究
基金资助:
湖北省自然科学基金(2014CFC1049);中国博士后科学基金(2014T70977)

Construction and screening of RPL23-siRNA interference fragments

PENG Wenmiao^1,2,ZHANG Zhimin¹,HU Meng¹,YU Lifang¹,ZHANG Bicheng¹,RAO Zhiguo¹,QIN Chuanrong^1,2

1.Department of Oncology,Wuhan General Hospital of PLA,Wuhan 430070,China;
2.Clinical Medicine College,Hubei University of Chinese Medicine

Received:2016-07-15 Online:2016-12-31 Published:2016-12-27

摘要/Abstract

摘要： 目的构建并筛选RPL23-siRNA干扰片段，为RPL23在胃癌防治中作用的研究提供基础。方法利用RNA干扰技术选择3条靶序列，通过脂质体转染化学合成的RPL23-siRNA干扰片段，用Real-time PCR及Western blot方法鉴定RPL23-siRNA对胃癌MKN45细胞中RPL23的干扰效率。结果实验分为5组，与Normal cell组、RPL23 control组相比，筛选出的3个RPL23-siRNA片段转染后，细胞中RPL23 mRNA及蛋白表达均明显下降(P＜0.01)，多重比较发现，RPL23-siRNA1组干扰效率明显高于RPL23-siRNA2、RPL23-siRNA3组(P＜0.01)。结论成功构建并筛选出RPL23-siRNA干扰片段，为后续研究提供了实验基础。

关键词: 核糖体蛋白L23, 小干扰RNA, RNA干扰

Abstract: Objective To construct and screen out the RPL23-siRNA interference fragments,providing the basis for the following experiments about the correlation with RPL23 and gastric cancer.Methods The RPL23-siRNA,synthesized chemically through lipofection,were selected from three target sequences by RNA interference and detected by real-time PCR and Western blot.Results Compared with normal cell group and RPL23 control group,the mRNA and protein expression of RPL23 in the other 3 interference groups were significantly decreased(P＜0.01).Multiple comparisons showed that the interference efficiency of RPL23-siRNA1 group was significantly higher than that of RPL23-siRNA2 group and RPL23-siRNA3 group(P＜0.01).Conclusion The RPL23-siRNA interference fragment can be successfully constructed and screened out,which provides the basis for the following experiments.

Key words: RPL23, siRNA, RNAi

中图分类号:

R735.2

彭文苗，张志敏，胡萌，余丽芳，章必成，饶智国，秦传蓉. RPL23-siRNA干扰片段的构建及筛选[J]. 实用肿瘤学杂志, 2016, 30(6): 487-491.

PENG Wenmiao,ZHANG Zhimin,HU Meng,YU Lifang,ZHANG Bicheng,RAO Zhiguo,QIN Chuanrong. Construction and screening of RPL23-siRNA interference fragments[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(6): 487-491.

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RPL23-siRNA干扰片段的构建及筛选

Construction and screening of RPL23-siRNA interference fragments

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