番茄红素对人肝癌HepG2细胞增殖和凋亡的影响

doi:10.11904/j.issn.1002-3070.2017.03.004

摘要/Abstract

摘要： 目的研究番茄红素(Lycopene,LP)对人肝癌HepG2细胞生长的影响并初探其机制。方法取对数生长期HepG2细胞设空白对照组、LP(5 μg/mL)组、LP(10 μg/mL)组、LP(20 μg/mL)组和顺铂(40 μg/mL)组,每组设10个复孔;各组分别给药干预48 h后,噻唑蓝(MTT)比色法测定细胞增殖抑制率,流式细胞术(FCM)检测细胞周期和细胞凋亡状况,RT-PCR法检测Bax mRNA和Bcl-2 mRNA表达,Western blot法检测Caspase-3蛋白表达。结果与空白对照组比较,经LP(10 μg/mL、20 μg/mL)或顺铂40 μg/mL干预48 h能够显著提高HepG2细胞增殖抑制率,延长细胞周期中G₀/G₁期而缩短G₂/M期,提高细胞凋亡率,上调促凋亡Bax mRNA表达并下调抑凋亡Bcl-2 mRNA表达,提高Bax/Bcl-2比值,上调Caspase-3蛋白表达,LP上述作用具有一定的剂量依赖性。结论 LP具有抑制人肝癌HepG2细胞增殖并促进其凋亡的作用,其机制可能与LP干预细胞周期分布和调节凋亡相关基因蛋白表达有关。

关键词: 番茄红素, 肝细胞癌, HepG2细胞, 增殖, 凋亡

Abstract: Objective The objective of this study was to investigate effects of lycopene(LP)on the proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and to explore its mechanism.Methods HepG2 cells in logarithmic growth phase were treated with 0,5,10,20 μg/mL of LP and 40 μg/mL of Cisplatin for 48 h.Ten replicates in each dose were designed in this study.After treatments,the cell viability was measured by MTT colorimetric assay.The distribution of cell cycle was detected by flow cytometry(FCM).The mRNA expression of Bax and Bcl-2 were measured by RT-PCR.The expression of Caspase-3 protein was explored by Western blot.Results The inhibition rate of HepG2 cells was significantly increased by 10 μg/mL and 20μg/mL of LP or 40 μg/mL of cisplatin when compared to the negative control group.The cell cycle of HepG2 cells were arrested at the G₀/G₁ phase and the apoptosis rate were significantly increased in comparison with the negative control group.The level of Bax mRNA expression was significantly increased and decreased in the expression of Bcl-2 mRNA.They were shown an increasing ratio of Bax/Bcl-2 and up-regulated Caspase-3 protein in HepG2 cells treated with LP.All effects in this study show a dose-dependent manner.Conclusion LP can inhibit the proliferation and promote the apoptosis in HepG2 cells.This mechanism may be contributed to arresting cell cycle and regulating gene expression related to apoptosis.

Key words: Lycopene, Human hepatocellular carcinoma HepG2 cells, Proliferation, Apoptosis

赵孟雷. 番茄红素对人肝癌HepG2细胞增殖和凋亡的影响[J]. 实用肿瘤学杂志, 2017, 31(3): 211-216.

ZHAO Menglei. Effects of lycopene on the proliferation and apoptosis in human hepatocellular carcinoma HepG2 cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 211-216.

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