TRAP1在食管癌发展中的作用及机制研究

doi:10.11904/j.issn.1002-3070.2018.06.007

摘要/Abstract

摘要： 目的探究肿瘤坏死因子受体相关蛋白1(TRAP1)在人食管癌进展中的作用及机制。方法用免疫组化检测人食管癌组织中TRAP1和S100A8的表达水平。在食管癌细胞系KYSE150中建立稳定下调TRAP1的细胞系, 用CCK-8检测细胞的增殖能力, Transwell检测细胞的转移能力, 流式细胞术检测细胞的凋亡。用实时荧光定量PCR检测TRAP1的下游基因E-Cadherin、N-Cadherin和S100A8的表达水平。结果 TRAP1在食管癌组织中的表达水平(55.0%)显著高于癌旁组织(11.7%), 并与S100A8具有一致性(χ²=4.141, P＜0.001)。得到稳定下调TRAP1的细胞系KYSE150-TRAP1, 与对照组细胞系KYSE150-control相比, KYSE150-TRAP1细胞系中TRAP1的表达水平下调85%(P＜0.05), 细胞的转移能力降低46%(P＜0.05), 细胞的增殖和凋亡无显著变化;E-Cadherin的表达水平升高19%(P＜0.05), S100A8的表达水平下降39%(P＜0.05)。结论 TRAP1在食管癌组织中过表达, 并通过调控S100A8的表达促进食管癌的转移。

关键词: 食管癌, 肿瘤坏死因子受体相关蛋白1, S100A8, 转移

Abstract: Objective The aim of this study was to investigate the role and mechanism of tumor necrosis factor receptor-related protein 1(TRAP1)in the progression of human esophageal cancer. Methods Immunohistochemistry was used to detect the expression of TRAP1 and S100A8 in human esophageal cancer tissues.A stably knocked-down TRAP1 cell line was established in the esophageal cancer KYSE150 cell line, the proliferation ability was detected by CCK-8, the transfer ability was detected by Transwell, and apoptosis was detected by flow cytometry.We conducted a gene profiling study to detect the expression of genes related to tumor progression.The expression of TRAP1 downstream genes-E-Cadherin, N-Cadherin and S100A8 was detected by Real-Time fluorescent quantitative PCR. Results The expression of TRAP1 in esophageal carcinoma was significantly higher than that in adjacent tissues and correlated with S100A8(χ²=4.141, P＜0.001).The KYSE150 cell line with down-regulated of TRAP1(KYSE150-TRAP1)was established, and the expression of TRAP1 was down-regulated by 85%, cell invaded ability was decreased by 46%, no changes of cell proliferation and apoptosis were observed, when compared to the KYSE150 control cells.The expression level of E-cadherin was increased by 19%, and the expression level of S100A8 was decreased by 39% in KYSE150-TRAP1 cells. Conclusion TRAP1 is overexpressed in esophageal carcinoma and promotes the metastasis of esophageal carcinoma by regulating S100A8 expression.

Key words: Esophageal cancer, TRAP1, S100A8, Metastasis

中图分类号:

R735.1

于芳, 赵培. TRAP1在食管癌发展中的作用及机制研究[J]. 实用肿瘤学杂志, 2018, 32(6): 515-519.

YU Fang, ZHAO Pei. The role and mechanism of TRAP1 in the development of esophageal cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 515-519.

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