细胞分裂周期7蛋白在肝癌组织的表达及其对肝癌细胞增殖和侵袭的影响

doi:10.11904/j.issn.1002-3070.2020.02.012

实用肿瘤学杂志 ›› 2020, Vol. 34 ›› Issue (2): 150-155.doi: 10.11904/j.issn.1002-3070.2020.02.012

细胞分裂周期7蛋白在肝癌组织的表达及其对肝癌细胞增殖和侵袭的影响

张从义¹, 崔逸峰¹, 李宝宝², 刘连新¹

1.哈尔滨医科大学附属第一医院普通外科(哈尔滨 150001);
2.哈尔滨医科大学附属第一医院神内科

收稿日期:2020-02-02 发布日期:2020-04-27
通讯作者: 刘连新,E-mail:liulianxin@medmail.com.cn
作者简介:张从义,男,(1994-),本科,从事肝脏肿瘤发生机制的研究。

Expression of cell division cycle 7 protein in hepatocellular carcinoma tissues and its effect on proliferation and invasion of hepatocellular carcinoma cells

ZHANG Congyi¹, CUI Yifeng¹, LI Baobao², LIU Lianxin¹

1.Department of General Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China;
2.Department of Neurology,The First Affiliated Hospital of Harbin Medical University

Received:2020-02-02 Published:2020-04-27

摘要/Abstract

摘要： 目的检测细胞分裂周期7(CDC7)蛋白在人肝癌组织的表达,探讨CDC7过表达对肝癌细胞增殖和侵袭的影响。方法实时定量聚合酶链式反应(Real-time PCR)检测CDC7 mRNA在肝癌组织和癌旁组织的表达,检测CDC7 mRNA在肝癌细胞系和正常肝脏细胞中的表达。Western blot检测CDC7蛋白在肝癌组织和癌旁组织中的表达,检测CDC7蛋白在肝癌细胞系和正常肝脏细胞中的表达水平。利用慢病毒载体构建稳定过表达CDC7的肝癌HCCLM3和SMMC 7721细胞株。细胞克隆实验和CCK-8法检测CDC7过表达对肝癌细胞增殖的影响。Transwell实验和划痕实验检测CDC7过表达对肝癌细胞侵袭迁移的影响。结果与癌旁组织相比,CDC7 mRNA和蛋白在肝癌组织中表达水平显著升高(P＜0.001);与正常肝细胞相比,肝癌细胞系的CDC7 mRNA和蛋白表达水平显著增高(P＜0.001);CCK-8法、细胞克隆实验说明CDC7过表达会明显增强肝癌细胞的增殖能力;划痕实验、Transwell实验说明CDC7过表达会明显提高肝癌细胞的侵袭能力。结论 CDC7蛋白在人肝癌组织高表达,其过表达促进肝癌细胞的增殖和侵袭能力。

关键词: 肝癌, 细胞分裂周期7蛋白, 增殖, 侵袭

Abstract: Objective The objectives of this study were to investigate the expression of cell division cycle 7 protein(CDC7)in hepatocellular carcinoma(HCC)tissues,and to explore the effect of CDC7 overexpressing on proliferation and invasion of hepatocellular carcinoma cells.Methods Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of CDC7 mRNA in liver cancer tissues and adjacent tissues,and the expression of CDC7 mRNA in liver tumor cell lines and normal liver cells.Western blot was used to detect the expression of CDC7 protein in liver cancer tissues and adjacent tissues,and to detect the expression of CDC7 protein in liver tumor cell lines and normal liver cells.Lentivirus vectors were used to construct stable overexpressing CDC7 hepatocellular carcinoma HCCLM3 and SMMC 7721 cell lines.Cell clone and cell counting kit-8(CCK-8)experiments were used to detect the effect of CDC7 overexpression on the proliferation of HCC cells.Scratch and Transwell assays were used to detect the effect of CDC7 overexpression on the invasion and migration of HCC cells.Results Compared with adjacent tissues,the expression of CDC7 at the levels of mRNA and protein was significantly increased in HCC tissues(P＜0.001);compared with normal liver cells,the expression levels of CDC7 at the levels of mRNA and protein were significantly increased in liver cancer cell lines(P＜0.001);CCK-8 experiments and cell cloning experiments showed that overexpression CDC7 significantly enhanced the proliferative ability of HCC cells;Scratch and Transwell experiments showed that overexpression CDC7 significantly improved the invasion ability of HCC cells.Conclusion CDC7 protein is highly expressed in human HCC tissues,and its overexpression promotes the proliferative and invasive capability of HCC cells.

Key words: Hepatocellular carcinoma, Cell division cycle 7 protein, Proliferative, Invasion

中图分类号:

R735.7

张从义, 崔逸峰, 李宝宝, 刘连新. 细胞分裂周期7蛋白在肝癌组织的表达及其对肝癌细胞增殖和侵袭的影响[J]. 实用肿瘤学杂志, 2020, 34(2): 150-155.

ZHANG Congyi, CUI Yifeng, LI Baobao, LIU Lianxin. Expression of cell division cycle 7 protein in hepatocellular carcinoma tissues and its effect on proliferation and invasion of hepatocellular carcinoma cells[J]. Journal of Practical Oncology, 2020, 34(2): 150-155.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2020.02.012

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I2/150

参考文献

1 Heimbach JK,Kuloik LM,Finn RS,et al.AASLD guidelines for the treatment of hepatocellular carcinoma[J].Hepatology,2018;67(1):358-380.
2 李诚,兰莉,杨超,等.2015-2017年哈尔滨市恶性肿瘤发病与死亡特征分析[J].实用肿瘤学杂志,2019,33(1):62-66.
3 Siegel RL,Miller KD,Jemal A,et al.Cancer statistics,2017[J].CA Cancer J Clin,2017,67(1):7-30.
4 Lozano R,Naghavi M,Foreman K,et al.Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010:a systematic analysis for the Global Burden of Disease Study 2010[J].Lancet,2012,380(9859):2095-2128.
5 Petrick JL,Kelly SP,Altekruse SF.Future of hepatocellular carcinoma incidence in the United States Forecast Through 2030[J].J Clin Oncol,2016,34(15):1787-1794.
6 Montagnoli A,Moll J,Colotta F.Targeting cell division cycle 7 kinase:a new approach for cancer therapy[J].Clin Cancer Res,2010,16(18):4503-4508.
7 Clarke LE,Fountaine TJ,Hennessy J,et al.Cdc7 expression in melanomas,Spitz tumors and melanocytic nevi[J].J Cutan Pathol,2009,36(4):433-438.
8 Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
9 Bonte D,Lindvall C,Liu H,et al.Cdc7-Dbf4 kinase overexpression in multiple cancers and tumor cell lines is correlated with p53 inactivation[J].Neoplasia,2008,10(9):920-931.
10 Spurgers KB,Gold DL,Coombes KR,et al.Identification of cell cycle regulatory genes as principal targets of p53-mediated transcriptional repression[J].J Biol Chem,2006,281(35):25134-25142.
11 Li X,Qian X,Jiang H,et al.Nuclear PGK1 alleviates ADP-dependent inhibition of CDC7 to promote DNA replication[J].Mol Cell,2018,72(4):650-660.
12 Jin S,Ma H,Yang W,et al.Cell division cycle 7 is a potential therapeutic target in oral squamous cell carcinoma and is regulated by E2F1[J].J Mol Med(Berl),2018,96(6):513-525.
13 Labib K.How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?[J].Genes Dev,2010,24(12):1208-1219.

[1]	王飞, 朱贲贲. 丙泊酚经HIF-1α信号通路对头颈鳞状细胞癌SCC-74细胞增殖、凋亡与侵袭的影响[J]. 实用肿瘤学杂志, 2020, 34(2): 120-125.
[2]	刘光霞, 赵连春, 陈芳, 贾乾, 高伟, 侯瞻, 卢亚敏. ZCCHC12对甲状腺细胞恶性转化的影响与作用机制[J]. 实用肿瘤学杂志, 2020, 34(2): 133-138.
[3]	王璞, 韦丽宾, 倪广晓. CCNB1基因通过调控PI3K/Akt信号通路对口腔鳞癌细胞增殖、侵袭和迁移的影响[J]. 实用肿瘤学杂志, 2020, 34(2): 144-149.
[4]	张巍, 姜晓东, 迟大鹏. WNT4在脑胶质瘤中甲基化下调及抑制恶性胶质瘤细胞增殖的研究[J]. 实用肿瘤学杂志, 2020, 34(2): 156-162.
[5]	赵楠楠, 秦文, 韩永焕, 鲁静, 李亚敏, 徐亚君. SOX11 抑制食管癌细胞增殖和迁移的机制研究[J]. 实用肿瘤学杂志, 2020, 34(1): 17-23.
[6]	赵云霞, 牛波. LncRNA NBAT1通过调控miR-3664-5p/Caspase 9分子轴对人乳腺癌细胞增殖的影响[J]. 实用肿瘤学杂志, 2020, 34(1): 24-29.
[7]	王鸣, 孙梯业. PFKFB4对胃癌细胞侵袭和迁移能力的影响及其作用[J]. 实用肿瘤学杂志, 2020, 34(1): 37-42.
[8]	詹琦, 周晟, 娄丽萍. EGFR,HER2和HER3在胆管癌中的表达及预后价值[J]. 实用肿瘤学杂志, 2019, 33(6): 491-496.
[9]	黄梨, 龙佑梅, 付义霞, 夏良斌. 紫檀芪对卵巢癌SKOV3细胞凋亡和糖酵解的影响及机制研究[J]. 实用肿瘤学杂志, 2019, 33(6): 502-507.
[10]	陈子印, 白艳春, 曹洋森, 李坚, 徐丽丽, 赵秋爽, 汪洋. 呼吸门控技术在肝癌大分割放射治疗方面的剂量学优势[J]. 实用肿瘤学杂志, 2019, 33(6): 536-539.
[11]	李素素, 周春祥. NKT细胞对肝癌免疫应答的双向调控作用[J]. 实用肿瘤学杂志, 2019, 33(6): 540-543.
[12]	来森艳, 胡发涌, 吴维, 李国东, 李小兰, 曹小年. DAB2IP稳定细胞系的构建及其对结直肠癌HT29细胞侵袭迁移能力的影响[J]. 实用肿瘤学杂志, 2019, 33(5): 385-389.
[13]	陈光浩, 方靖萱, 彭素娟, 严炜薇, 王福海, 陈承哲, 陈泗川, 潘建基, 邱素芳. PNCK对鼻咽癌细胞增殖和凋亡作用的研究[J]. 实用肿瘤学杂志, 2019, 33(5): 397-401.
[14]	周瑶瑶, 陈凤云, 屠文骆. 棕榈酸在体外通过促进凋亡作用抑制宫颈癌Hela细胞增殖的研究[J]. 实用肿瘤学杂志, 2019, 33(5): 402-406.
[15]	宋玲, 付琼. 紫云英苷通过上调PHD2抑制缺氧诱导的卵巢癌细胞增殖和侵袭[J]. 实用肿瘤学杂志, 2019, 33(5): 407-414.

细胞分裂周期7蛋白在肝癌组织的表达及其对肝癌细胞增殖和侵袭的影响

Expression of cell division cycle 7 protein in hepatocellular carcinoma tissues and its effect on proliferation and invasion of hepatocellular carcinoma cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价