Nultin-3联合顺铂对口腔鳞状细胞癌细胞的抑制作用及机制研究

doi:10.11904/j.issn.1002-3070.2018.06.002

实用肿瘤学杂志 ›› 2018, Vol. 32 ›› Issue (6): 488-492.doi: 10.11904/j.issn.1002-3070.2018.06.002

Nultin-3联合顺铂对口腔鳞状细胞癌细胞的抑制作用及机制研究

施俊¹, 倪小兵¹, 毛敏¹, 彭兴春²

1.湖北医药学院附属太和医院口腔科(十堰 442000);
2.湖北医药学院基础医学院

收稿日期:2018-03-03 出版日期:2018-12-28 发布日期:2018-12-27
通讯作者: 彭兴春, E-mail:pxcun118@163.com
作者简介:施俊, 女, (1984-), 硕士, 主治医师, 从事口腔相关疾病的研究。
基金资助:
湖北省教育厅科研计划项目(编号:B2018109);湖北医药学院2017年教学研究项目(编号:2017035;2017032)

Inhibitory effect and mechanism of Nultin-3 combined with cisplatin on oral squamous cell carcinoma

SHI Jun¹, NI Xiaobing¹, MAO Min¹, PENG Xingchun²

1.Department of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China;
2.School of Basic Medical Sciences, Hubei University of Medicine

Received:2018-03-03 Online:2018-12-28 Published:2018-12-27

摘要/Abstract

摘要： 目的探讨MDM2抑制剂Nultin-3联合顺铂对口腔鳞状细胞癌Tca8113细胞的抑制作用及其机制。方法将人口腔鳞状细胞癌Tca8113细胞分为对照组、顺铂组、Nultin-3组及Nultin-3联合顺铂组(联合组), 采用噻唑蓝(MTT)法分别检测各组Tca8113细胞处理48 h后的增殖率;采用蛋白质印迹法检测各组细胞MDM2、p53及凋亡相关蛋白Caspase-3和Caspase-9的表达。结果联合组处理后口腔鳞状细胞癌Tca8133细胞的增殖率明显低于其它各组, 差异具有统计学意义(P＜0.05)。联合组Caspase-9、Caspase-3及p53蛋白的相对表达量明显高于顺铂组和Nultin-3组(P＜0.05);此外, 联合组MDM2蛋白的相对表达量显著低于顺铂组和Nultin-3组(P＜0.05)。结论 Nultin-3联合顺铂作用于口腔鳞癌细胞Tca8113时具有协同增效作用。Nultin-3通过调控MDM2-p53信号通路并上调促凋亡蛋白Caspase-9及Caspase-3的表达水平, 以达到增强顺铂抑制口腔鳞状细胞癌的作用, 从而为相关临床研究及治疗提供坚实的理论基础。

关键词: Nultin-3, 顺铂, Tca8113细胞, MDM2, p53

Abstract: Objective The Objective of this study was to investigate the inhibitory effect of MDM2 inhibitor Nultin-3 combined with cisplatin on human oral squamous cell carcinoma(OSCC)Tca8113 cells and its mechanism. Methods Human OSCC Tca8113 cells were treated with Nultin-3, cisplatin, Nultin-3 combined with cisplatin, or vehicle control groups.The proliferation of Tca8113 cells was determined by thiazolyl blue(MTT)assay.The expression of MDM2, P53, Caspase-9 and Caspase-3 protein was determined by Western blot. Results The proliferative rate of OSCC ca8133 cells treated with Nultin-3 combined with cisplatin was significantly lower than that of other groups(P＜0.05).The relative expression of Caspase-9, Caspase-3 and P53 protein in the Nultin-3 combined with cisplatin was significantly higher than those of the Nultin-3 and cisplatin alone groups(P＜0.05).In addition, the relative expression of MDM2 protein in the Nultin-3 combined with cisplatin group was significantly lower than that of the cisplatin and Nultin-3 alone groups(P＜0.05). Conclusion Nultin-3 combined with cisplatin has synergistic effect on oral squamous cell carcinoma Tca8113 cells.Nultin-3 regulates the MDM2-p53 signaling pathway and up-regulates the expression of proapoptotic proteins Caspase-9 and Casapase-3 to enhance the inhibition of cisplatin on oral squamous cell carcinoma, providing a solid theoretical basis for clinical research and treatment.

Key words: Nultin-3, Cisplatin, Tca8113 cells, MDM2, P53

中图分类号:

R782.05

施俊, 倪小兵, 毛敏, 彭兴春. Nultin-3联合顺铂对口腔鳞状细胞癌细胞的抑制作用及机制研究[J]. 实用肿瘤学杂志, 2018, 32(6): 488-492.

SHI Jun, NI Xiaobing, MAO Min, PENG Xingchun. Inhibitory effect and mechanism of Nultin-3 combined with cisplatin on oral squamous cell carcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 488-492.

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Nultin-3联合顺铂对口腔鳞状细胞癌细胞的抑制作用及机制研究

Inhibitory effect and mechanism of Nultin-3 combined with cisplatin on oral squamous cell carcinoma

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