miR-34a对结肠癌细胞SW480增殖、迁移和侵袭能力的影响及机制探讨

doi:10.11904/j.issn.1002-3070.2017.03.001

Abstract

Abstract: Objective The objective of this study was to investigate effects of miR-34a on the proliferation,invasion and migration of colon cancer SW480 cell and its possible mechanism.Methods miR-34a overexpressed lentivirus and empty virus vector were transfected into SW480 cells and untreated cells were used as blank control group.Real-time PCR was used to detect the expression of miR-34a in each group.The cell proliferation was detected by CCK8 assay.The cell migration and invasion ability were detected by wound healing and transwell assays.The expression of E-cadherin and Vimentin protein was detected by Western blotting.Results Compared with the empty virus vector group and the blank control group,the expression of miR-34a was increased in the transfected cells,and the cell proliferation efficiency,invasion and migration ability were decreased in the transfected cells(P＜0.05).miR-34a significantly increased the expression of E-cadherin protein and decreased Vimentin protein expression in the transfected cells.Conclusion miR-34a can inhibit the proliferation,invasion and migration of colon cancer SW480 cells,and affect the expression of E-cadherin and Vimentin.MR-34a is expected to be a potential molecular target for the metastasis and recurrence of colorectal cancer.

Key words: Colorectal cancer, miR-34a, EMT, Proliferation, Invasion

CLC Number:

R735.3

HAO Xiaojun,WANG Chuanzhuo,ZHAO Xiangxuan,XIN he,LIU Zhaoyu. Effects of miR-34a on proliferation,migration and invasion of colon cancer SW480 cell and its possible mechanism[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 193-198.

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Effects of miR-34a on proliferation,migration and invasion of colon cancer SW480 cell and its possible mechanism

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