蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究

doi:10.11904/j.issn.1002-3070.2017.05.001

Abstract

Abstract: Objective The objective of this study was to investigate the effect of dauricine on the sensitivity of 5-fluorouracil(5-FU)in human breast cancer MCF-7 cells.Methods MCF-7 cells were treated with 2.5 μg/mL of dauricine,50 μg/mL of 5-FU,or 2.5 μg/mL of dauricine with 50 μg/mL of 5-FU,the cell proliferation was measured by MTT assay.The cell migration was determined by Transwell assay;The cell apoptosis was detected by DAPI staining;The expression of cyclin D1 and Bcl-2 gene was examined by Western blot.Results The results showed that the combination of subthreshold concentration of dauricine enhanced the inhibitory effect of 5-FU on proliferation in MCF-7 cells.The combined use of subcutaneous concentration of dauricine further aggravated the inhibitory effect of 5-FU on cell migration.The combination of subcapsular dauricine enhanced the induction of apoptosis by 5-FU.The combination of dauricine with 5-FU could inhibit the expression of cyclin D1 and Bcl-2 protein in MCF-7 cells.Conclusion Dauricine can effectively enhance the sensitivity of 5-FU in human breast cancer MCF-7 cells.

Key words: Dauricine, 5-Fluorouracil, Breast cancer, MCF-7 cells

CLC Number:

R737.9

LI Hongyang, SUN Liang, JIANG Xing, YUAN Guangjun, LIU Yan. Dauricine enhances the sensitivity of 5-fluorouracil in human breast cancer MCF-7 cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(5): 385-389.

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Dauricine enhances the sensitivity of 5-fluorouracil in human breast cancer MCF-7 cells

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