Abstract: Objective The aim of this study was to investigate the anti-tumor effect on sequential injection of heterogeneic lymphocyte(HL)and autogeneic lymphocyte(AL). Methods The HL was prepared by using CC3HF1 mice as feeders.CB6F1 mice were used as recipients, and Hepa1-6 cells were inoculated into the recepients′ groin subcutis.A cryoprecipitate was extracted from mouse plasma by freeze-thaw method to prepare fibrin Glue(FG);FG was combined with HL or AL to be FG-HL or FG-AL.The experimental treatment consisted of two stages.At first stage(15 d), FG-HL were injected on the surface of the tumor-bearing tissue of the recipients as the experimental group, and FG-phosphate buffer saline(FG-PBS)were injected on the surface of the tumor-bearing tissue of the rest recipients as the control group.The immunological factors such as tumor cell killing rate of the spleen lymphocytes and numbers of lymphocytes, CD8⁺T and NK in the two groups were detected, respectively.At later stage(10 d), a part of mice were randomly selected from the experimental and control groups, and the lymphocytes(AL)were used to form FG-AL, which were injected on the surface of tumor-bearing tissues in the rest of mice.Tumors in mice of the two groups were compared for tumor volume and tumor inhibition rate. Results The tumor cell killing rate of AL in the experimental group(26.70±7.22) was significantly higher than that in the control group(5.70±2.68)(P＜0.01).Numbers of mouse spleen lymphocytes, CD8⁺T cells and NK cells were significantly higher than the corresponding values of the control group(P＜0.05).After the two-stage treatment, the average tumor volume of the experimental group[(1.20±0.33)cm³]was significantly smaller than that of control group[(2.05±0.37)cm³](P＜0.01).The tumor inhibition rate in the experiment group was 41.5% when compared to the control group. Conclusion Local injections of FG-HL followed by FG-AL can significantly inhibit the growth of transplanted tumor in mice;it is expected to become an anti-tumor biological therapy.

Key words: Lymphocyte, Injection, Hepatocarcinoma, Fibrin glue