TSP-1基因对骨肉瘤血管生成的影响及其作用机制

doi:10.11904/j.issn.1002-3070.2019.02.002

Abstract

Abstract: Objective The aim of this study was to investigate the effect of TSP-1 gene on angiogenesis in human osteosarcoma and its mechanism of action. Methods MG-63 cells were transfected with constructing pBPLV-shRNA-TSP-1 vector and pBPLV-TSP-1 expression vector.Cell viability was measured by CCK8,and its invasive ability was measured by Transwell assay.The expression of CD36 in intracells was detected by immunofluorescence.The expression levels of TSP-1,CD36,p38MAPK,VEGF,VEGFR-1,EGF and PDGF were detected in MG-63 cells by qRT-PCR and Western blot. Results The cell viability and invasion ability were significantly increased after transfected pBPLV-TSP-1 vector compared with the empty vector group(P＜0.05),and significantly decreased after transfected pBPLV-shRNA-TSP-1 vector(P＜0.05).The expression of TSP-1,EGF,P38,PDGF,VEGF and VEGFR-1 at mRNA and protein levels was significantly increased after transfection pBPLV-TSP-1(P＜0.05),and significantly decreased after transfection pBPLV-shRNA-TSP-1 vector(P＜0.05). Conclusion TSP-1 gene can promote the proliferation and invasion of MG-63 cells,and promote the formation of human osteosarcoma,indicating its mechanism related to the increase of growth factors EGF,VEGF,PDGF and activation of P38-MAPK pathway.

Key words: Osteosarcoma, TSP-1, MG-63, P38-MAPK pathway

CLC Number:

R73-37

ZHU Huanye, JIAN Yuekui, LI Bo, GAO Gan, TIAN Zhen, SHU Man. Effect of TSP-1 gene on angiogenesis of osteosarcoma and its mechanism[J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(2): 103-109.

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Effect of TSP-1 gene on angiogenesis of osteosarcoma and its mechanism

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