ZNF436在胃癌中的表达及调控WNT/β-catenin对胃癌细胞迁移及侵袭的影响

doi:10.11904/j.issn.1002-3070.2019.03.006

Abstract

Abstract: Objective The aim of this study was to investigate the relative expression of zinc finger protein ZNF436 in human gastric cancer cells and tissues and its mechanism of ZNF436 on migration and invasion of gastric cancer MKN-28 cells.Methods RT-qPCR and immunohistochemistry were used to determine the expression of ZNF436 at the levels of mRNA and protein in human gastric cancer cells,gastric cancer and matched paracancerous tissues,and normal human gastric tissues.ZNF436 expression and prognostics were analyzed through online data.The ZNF436-siRNA(experimental group)and NC-siRNA(control group)plasmids were transfected into MKN028 cells.RT-qPCR was used to confirm the knockout efficiency of ZNF436 after transfection for 48 hours.The transwell assay was used to analyze the effect of ZNF436 on cell migration and invasion after knockout ZNF436.The effect of ZNF436 on cell metastasis was verified by nude mice metastasis experiments.The effect of ZNF436 on WNT/β-catenin signaling pathway was verified by immunoblotting.Results Compared with normal gastric epithelial cells,ZNF436 was highly expressed in human gastric cancer cells(P＜0.001).ZNF436 was highly expressed in gastric cancer tissues compared with adjacent normal tissues and normal human gastric tissues(P＜0.001).The expression of ZNF436 was associated with the prognosis of gastric cancer from online data(P=0.0028);the high expression of ZNF436 gene,the worse prognosis of patients(P＜0.001);When compared with the control group,the migration and invasion ability in the experimental group was significantly inhibited(P＜0.001);the size and volume of metastasis in nude mice in the experimental group were significantly lower than those in the experimental group(P＜0.001).After the low expression of ZNF436,the WNT/β-catenin signaling pathway and its downstream cytokines were significantly inhibited.Conclusion ZNF436 is up-regulated as a potential oncogene in human gastric cancer and participates in the progression of gastric cancer by promoting WNT/β-catenin signaling pathway.

Key words: Gastric cancer, ZNF436, Oncogene, Cell migration, Invasion, WNT/β-catenin

CLC Number:

R735

QIN Wen,ZHAO Nannan,HAN Yonghuan,XU Yajun. Expression of ZNF436 in gastric cancer and its effect on the migration and invasion of gastric cancer cells by WNT/β-catenin[J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 222-227.

References 1

Golemis EA,Scheet P,Beck TN,et al.Molecular mechanisms of the preventable causes of cancer in the United States[J].Genes Dev,2018,32(13):868-902.2 Roy RK,Hoppe MM,Sivastava S,et al.CEACAM6 is upregulated by Helicobacter pylori CagA and is a biomarker for early gastric cancer[J].Oncotarget,2016,7(34):55290-55301.3 Chen XZ,Chen H,Castro FA,et al.Epstein-barr virus infection and gastric cancer:a systematic review[J].Medicine(Baltimore),2015,94(20):e792.4 Shao XJ,Miao MH,Xue J,et al.The down-regulation of MicroRNA-497 contributes to cell growth and cisplatin resistance through PI3K/Akt pathway in osteosarcoma[J].Cell Physiol Biochem,2015,36(5):2051-2062.5 Chen G,Fang T,Huang Z,et al.MicroRNA-133a inhibits osteosarcoma cells proliferation and invasion via targeting IGF-1R[J].Cell Physiol Biochem,2016,38(2):598-608.6 时志龙,孙斌,陈磊,等.快速大容量尾静脉注射法建立肝细胞癌小鼠模型[J].中国肿瘤生物治疗杂志,2014,21(5):574-577.7 Wenger Y,Buzgariu W,Galliot B,et al.Loss of neurogenesis in Hydra leads to compensatory regulation of neurogenic and neurotransmission genes in epithelial cells[J].Philos Trans R Soc Lond B Biol Sci,2016,371(1685):20150040.8 DLi Y,Du X,Li F,et al.A novel zinc-finger protein ZNF436 suppresses transcriptional activities of AP-1 and SRE[J].Mol Biol Evol,2015,32(8):1928-1947.9 Harris JM,Nguyen PP,Patel MJ,et al.Functional diversification of hsp40:distinct j-protein functional requirements for two prions allow for chaperone-dependent prion selection[J].PLoS Genet,2014,10(7):e1004510.10 Chen X,Yao H,Yao L,et al.Selenium deficiency influences the gene expressions of heat shock proteins and nitric oxide levels in neutrophils of broilers[J].Biol Trace Elem Res,2014,161(3):334-340.11 Wisén S,Bertelsen EB,Thompson AD,et al.Binding of a small molecule at a protein-protein interface regulates the chaperone activity of hsp70-hsp40[J].ACS Chem Biol,2010,5(6):611-622.12 Shan Z,Wei Z,Shaikh ZA.Suppression of ferroportin expression by cadmium stimulates proliferation,EMT,and migration in triple-negative breast cancer cells[J].Toxicol Appl Pharmacol,2018,17(356):36-43.13 Chistiakov DA,Chekhonin VP.Circulating tumor cells and their advances to promote cancer metastasis and relapse,with focus on glioblastoma multiforme[J].Exp Mol Pathol,2018,105(2):166-174.

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Expression of ZNF436 in gastric cancer and its effect on the migration and invasion of gastric cancer cells by WNT/β-catenin

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