TMEM45A在肾透明细胞癌中的表达及作用机制研究

doi:10.11904/j.issn.1002-3070.2019.04.006

Abstract

Abstract: Objective The Objective of this study was to investigate the expression and function of TMEM45A(transmembrane protein 45A)in clear cell renal cell carcinoma(ccRCC).Methods The data of TMEM45A in Oncomine database were extracted by R language.The relationship between the expression level of TMEM45A and the stage or survival time of ccRCC was analyzed by GEPIA database.qRT-PCR and Western blot were used to detect the expression of TMEM45A in ccRCC tissues and renal cell carcinoma cell lines.After transfection of TMEM45A siRNA,the low expression ofTMEM45A in Caki-1 cells was confirmed by qRT-PCR and Western blot.Cell proliferation after knockdown TMEM45A was analyzed in Caki-1 cells by CCK8.The mechanism of action in the low expression of TMEM45A inhibited cell proliferation was analyzed in Caki-1 cells by qRT-PCR and Western blot.Results A total of 384 TMEM45A-related studies were collected in the Oncomine database,with 35 statistically significant differences,25 of them elevated and 10 decreased.Four studies were associated with ccRCC with a total of 115 samples.The expression of TMEM45A was significantly increased in ccRCC(P＜0.05).It was also found that the high expression of TMEM45A was closely associated with high ccRCC stage and poor prognosis(P＜0.05).When compared with the normal kidney tissues,TMEM45A mRNA was significantly increased in ccRCC tissues(P＜0.05).The expression of TMEM45A at levels of mRNA and protein in Caki-1 and 786-0 cells was higher than that in normal renal tubular epithelial HK-2 cells.After transfection with TMEM45A siRNA for 48h and 72h,the proliferation of Caki-1 cells was significantly decreased(P＜0.001).At the same time,it was found that the expression of PCNA and cyclin D1 at levels of mRNA and protein was significantly decreased(P＜0.05).Conclusion The expression of TMEM45A is elevated in ccRCC and is associated with ccRCC staging and prognosis.It may be involved in the proliferation of renal carcinoma cells by regulating PCNA and Cyclin D1.

Key words: Clear cell renal cell carcinoma, TMEM45A, Oncomine database, GEPIA database, Proliferation

CLC Number:

R737.11

YAN Ruodong, REN Shan, SUI Wenyin, PIAO Renjing. Expression and mechanism of TMEM45A in renal clear cell carcinoma[J]. Journal of Practical Oncology, 2019, 33(4): 317-322.

References

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Expression and mechanism of TMEM45A in renal clear cell carcinoma

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