lncRNA BLACAT1靶向LEMD1促进结直肠癌细胞的增殖和转移

doi:10.11904/j.issn.1002-3070.2020.05.006

Abstract

Abstract: Objective The mechanism of long-chain non-coding RNA(lncRNA)BLACAT1 was studied in the occurrence and development of colorectal cancer cells.Methods StarBase was used to analyze the expression of lncRNA BLACAT1 in colorectal cancer tissues and adjacent tissues by the TCGA database.qRT-PCR was used to detect the expression of lncRNA BLACAT1 in tumor and adjacent tissues of 10 colorectal cancer patients.The expression of lncRNA BLACAT1 at the mRNA level was detected in normal colorectal epithelial FHC cells and several colorectal cancer cell lines of SW620,SW480,HT-29,LoVo and HCT116.The high expression of lncRNA BLACAT1 was screened in these cell lines.The effects of lncRNA BLACAT1 knockdown on proliferation,migration and invasion were verified in colorectal cancer cell lines.Targets of lncRNA BLACAT1 predicted by starBase online database were verified by qPCR and western blot.The correlation between lncRNA BLACAT1 and its target gene was validated by dual luciferase reporter gene.Finally,effects of target genes of lncRNA BLACAT1 on cell proliferation,migration and invasion of colorectal cancer cells were detected.Results Both database analysis and colorectal cancer patient detection results showed that the expression of lncRNA BLACAT1 in tumor tissues was significantly higher than that in adjacent tissues(P＜0.001).The expression of lncRNA BLACAT1 mRNA was the highest in colorectal cancer SW620 cells(45.180±0.892,t=110.6,P＜0.001).Knockdown of lncRNA BLACAT1 could inhibit the proliferation(P＜0.001),migration(P＜0.001)and invasion(P＜0.001)ability of SW620 cells.The results of qPCR and Western blot and database analysis showed that there was a positive correlation between the expression of lncRNA BLACAT1 and LEMD1(r=0.778,P＜0.001).The dual luciferase reporter gene experiment proved that LEMD1 was the target gene of lncRNA BLACAT1.lncRNA BLACAT1 up-regulated the expression of LEMD1 and promoted the proliferation(P＜0.001),migration(P＜0.001)and invasion(P＜0.001)of SW620 cells.Conclusion lncRNA BLACAT1 targeting LEMD1 promotes the proliferation and metastasis of colorectal cancer SW620 cells.

Key words: Colorectal cancer, lncRNA BLACAT1, LEMD1, Proliferation, Metastasis

CLC Number:

R735.3

DONG Manku, JIANG Fuquan, LIN Haiguan, SUN Xiaomin, LIU Jingxin, HU Gang, YANG Jianwu. LncRNA BLACAT1 targeting LEMD1 promotes the proliferation and metastasis of colorectal cancer cells[J]. Journal of Practical Oncology, 2020, 34(5): 418-424.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.05.006

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I5/418

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LncRNA BLACAT1 targeting LEMD1 promotes the proliferation and metastasis of colorectal cancer cells

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