Abstract:

Objective To investigate the inhibition and apoptotic effect of trihydroxybenzoic acid on HL-60 cells.Methods MTT-based cytotoxicity assay was used to test the HL-60 cells proliferation.The apoptotic cells were detected by AO fluorescent staining and flow cytometry;Results TBA inhibited HL-60 cells proliferation in a dose-dependent manner,HL-60 cells treated with TBA for 48h,AO fluorescent staining results showed that part of cell cytoplasm condensed,the volume was narrow,nuclear shrinkage,chromatin increased,more brightly fluorescent,condensed to form dense green fluorescent apoptotic features such as.Compared with control group(2.53±0.56)%,the percentage of early apoptotic cells was(28.24±5.24)%,the difference was significant(P＜0.05).Conclusion TBA inhibited the proliferation of HL-60 cells by inducing apoptosis.

Key words: 3,4,5-trihydroxybenzoic acid, HL-60 cells, Apoptosis