miR-182对直肠癌细胞增殖、侵袭及Foxo3a/Wnt/β-catenin通路的调节作用

doi:10.11904/j.issn.1002-3070.2020.04.007

Abstract

Abstract: Objective The aims of this study were to determine the effects of miR-182 on the proliferation and invasion of rectal cancer cells,and the regulation of Foxo3a/Wnt/β-catenin pathway. Methods Rectal cancer tissues and adjacent tissues that were surgically removed from June 2016 to July 2019 were collected,and human rectal cancer HT29,SW620,and HCT-116 cell lines and normal intestinal epithelial HIEC cells were cultured to detect the expression of miR-182.SW620 cells were treated in these following groups:the control group was treated with RPMI-1640 without drugs;the NC group was transfected with NC mimics and the miR-182 group was transfected with miR-182 mimics.The MTS assay was used to detect proliferation activity,Transwell was used to detect invasion activity,and Western blot was used to detect the expression of Foxo3a/Wnt/β-catenin pathway molecules in SW620 cells. Results The expression of miR-182 in rectal cancer tissues(2.14±0.55)was significantly higher than that in adjacent tissues(1.06±0.24)(P＜0.05);the expression of miR-182 in HT29,SW620 and HCT-116 cells were significantly higher than that in HIEC cells(P＜0.05),and the expression of miR-182 in SW620 cells increased most significantly;Proliferation activity in the miR-182 group(0.92±0.15)was significantly higher than that in the control group(0.52±0.08)and the NC group(0.55±0.07)(P＜0.05);The number of invasion in the miR-182 group(39.49±7.61)was significantly more than that of the control group(23.25±5.85)and the NC group(21.84±4.77)(P＜0.05);The number of migration in the miR-182 group(44.12±9.29)was significantly more than that in the control group(29.39±6.18)and the NC group(32.83±6.68)(P＜0.05);The expression of Foxo3a in the miR-182 group(0.36±0.07)was significantly lower than that in the control group(0.83±0.15)and the NC group(0.86±0.12)(P＜0.05);The expression of Wnt2 in the miR-182 group(0.86±0.15)was significantly higher than that in the control group(0.62±0.09)and the NC group(0.58±0.07)(P＜0.05);The expression of β-catenin in the miR-182 group(0.79±0.15)was significantly higher than that in the control group(0.41±0.07)and the NC group(0.45±0.08)(P＜0.05). Conclusion miR-182 can promote the proliferation and invasion of rectal cancer cells,and its mechanism may be related to targeting Foxo3a/Wnt/β-catenin pathway.

Key words: Rectal cancer, miR-182, Proliferation, Invasion, Foxo3a/Wnt/β-catenin pathway

CLC Number:

R656

CHEN Can, ZHANG Yanling, LIU Xiaoqing, RAN Fengwei. The effect of miR-182 on the proliferation and invasion of rectal cancer cells and regulation of Foxo3a/Wnt/β-catenin pathway[J]. Journal of Practical Oncology, 2020, 34(4): 328-333.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.04.007

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I4/328

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The effect of miR-182 on the proliferation and invasion of rectal cancer cells and regulation of Foxo3a/Wnt/β-catenin pathway

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