SNG162通过上调ER-α36表达激活胶质母细胞瘤膜起始的雌激素信号通路

doi:10.11904/j.issn.1002-3070.2025.03.002

Abstract

Abstract: Objective The aim of this study was to explore the effects of changes in the estrogen receptor alpha 36(ER-α36)expression on the proliferation and membrane-initiated estrogen signaling in glioblastoma U251 cells. Methods The expression and localization of ER-α36 and EGFR glioblastoma U87 cells and U251 cells were determined by immunofluorescence,qRT-PCR and Western blot.The effect of upregulating ER-α 36 on U251 cell proliferation and estrogen signaling pathway activity by low concentrations of 100 pmol/L icariin isomer(SNG162)was detected by MTT assay and Western blot. Results ER-α36 and EGFR were co-expressed in the cell membrane of glioblastoma.Compared with DMSO(control group),the expression ER-α36(P＜0.01)and EGFR increased in U251 cells treated with SNG162(P＜0.05);Further experments also found that low concentrations of SNG162 increased the expression of cycle related proteins-cyclin D1,cyclin B,cyclin E and CDK4(P＜0.01),and enhanced the proliferative ability of U251 cells(P＜0.05). Conclusion The low concentration of SNG162 upregulates the expression of ER-α36,activates the estrogen-mediated ERK1/2 MAPK,p38 MAPK,and EGFR/Src signaling pathways,promotes glioblastoma proliferation,and activates the membrane initialized estrogen signaling pathway.

Key words: Glioblastomas, Estrogen receptor-alpha36, Estrogen, Icariin isomer

CLC Number:

R739.41

ZHAO Entong, GUAN Xin, LI Hongyan, QIU Yunting, ZHANG Bingqiang, CHEN Mengmeng, ZOU Wei, QU Chao. SNG162 activates the estrogen signaling pathway at the membrane initiation of glioblastoma by upregulating ER-α36 expression[J]. Journal of Practical Oncology, 2025, 39(3): 177-183.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2025.03.002

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2025/V39/I3/177

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SNG162 activates the estrogen signaling pathway at the membrane initiation of glioblastoma by upregulating ER-α36 expression

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