MicroRNA-27a对U251胶质瘤细胞的影响

doi:10.11904/j.issn.1002-3070.2016.04.011

Abstract

Abstract: Objective To discuss the effect of microRNA-27a on U251 glioma cells.Methods Overexpression or inhibition of miR-27a in U251 glioma cells were done by transient transfection of miR-27a mimics or AMO-27a in vitro.Cell viability was detected by MTT assay.Invasion ability of U251 was detected by transwell invasion assay.The level of miR-27a and PPARγ were detected by real-time PCR.Results Under inhibition of miR-27a condition,the proliferation and invasiveness of U251 glioma cells were decreased.The level of PPARγ was significantly increased,whereas the level of miR-27a was decreased.Overexpression of miR-27a increased the proliferation and invasiveness of U251 glioma cells and decreased the level of PPARγ.Conclusion Inhibition of miR-27a is benefit for inhibiting the proliferation and invasiveness of U251 glioma cells.

Key words: MicroRNA-27a, Glioma, PPARγ, Proliferation, Invasiveness

CLC Number:

R739.41

LIU Guojun,ZHANG Jun,FAN Yongchun,WANG Qingsuo,LI Wei. The effect of microRNA-27a on U251 glioma cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(4): 346-350.

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The effect of microRNA-27a on U251 glioma cells

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