原苏木素A对胃癌SGC-7901细胞系放疗增敏作用的研究

doi:10.11904/j.issn.1002-3070.2017.06.004

Abstract

Abstract: Objective In this study,Protosappanin A,Caesalpinia Sappan L extract and Cisplatin were combined with radiotherapy in gastric cancer cell SGC-7901 to investigate whether the Protosappanin A could increase radiosensitivity(SER)in gastric cancer SGC-7901 cells.This will be medication to create new areas of innovation in the future.Methods The cell proliferation of SGC-7901 cells was detected by MTT assay.The relationship between the effect of the Protosappanin A on cell proliferation and the time of action was determined.Caesalpinia Sappan L extract and Cisplatin were as controls.The fitted cell survival curve and clonal formation assays were used to determine the SER to analyze the sensitizative effect of Protosappanin A.Results Protosappanin A could inhibit the growth of SGC-7901 cells,and its inhibitory effect is relatively weak.Its cytotoxicity has a time-and concentration-dependent manner.Cellular morphological changes were observed accompanying with increased concentrations and time treatments of Protosappanin A.Clonal formation experiment showed that the Protosappanin A significantly increased the radiosensitivity of SGC-7901 cells when compared to the radioactive group.They showed a statistically difference.Conclusion The inhibitory effect of the Protosappanin A on SGC-7901 cells in a concentration and time-dependent manner.Protosappanin A combined radiotherapy can improve the radiosensitization of cells,both of which may have synergistic anti-tumor effect.

Key words: Protosappanin A, Caesalpinia Sappan L extract, Gastric cancer, Radiotherapy, Radiosensitivity

CLC Number:

LIU Guohui, GU Anxin, YIN Hongtao, CAO Yang, HE Yunlong, WANG Chunbo, E Mingyan. Protosappanin A increases the sensitivity of gastric cancer SGC-7901 cells to radiotherapy[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(6): 500-505.

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Protosappanin A increases the sensitivity of gastric cancer SGC-7901 cells to radiotherapy

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