lncRNA-ATB介导的上皮间质转化在胃癌进展中的机制研究

doi:10.11904/j.issn.1002-3070.2022.03.003

Abstract

Abstract: Objective The objective of this study was to investigate the possible mechanism of lncRNA-ATB mediated epithelial-mesenchymal transition(EMT)in the progression of gastric cancer. Methods The downstream binding molecules of lncRNA-ATB were predicted by bioinformatics analysis and verified by luciferase reporter gene.A total of 56 gastric cancer and adjacent tissue specimens from the 980th Hospital of the Joint Logistics Support Force were collected from January 2017 to December 2019.The levels of lncRNA-ATB and the downstream binding molecules in gastric cancer and adjacent tissues as well as their relationship were analyzed by qRT-PCR.Its correlation with clinicopathological features of gastric cancer was also analyzed.The expression of miR-200a,β-catenin,vimentin and E-cadherin was confirmed by qRT-PCR and Western blot in gastric cancer BGC-823 cells before and after knockdown of lncRNA-ATB. Results Bioinformatics analysis showed that lncRNA-ATB had a direct binding site to miR-200a,and miR-200a could directly bind to β-catenin,which was verified by luciferase reporter gene.The expression of lncRNA-ATB in gastric cancer tissues was significantly higher than that in adjacent tissues(P＜0.001),and the expression of miR-200a in gastric cancer was significantly lower than that in adjacent tissues,the difference was statistically significant(P＜0.001).There was a negative correlation between the levels of lncRNA-ATB and miR-200a in gastric cancer tissues(r=-0.317,P=0.017).The expression of lncRNA-ATB in the stage III and IV gastric cancer was significantly higher than that in the stage I and II gastric cancer,and the expression of lncRNA-ATB in patients with the lymph node metastasis positive group,vascular tumor thrombus positive group and tumor poorly differentiated group was significantly higher than that in the negative group,and the difference was statistically significant(P＜0.001).The expression of miR-200a in the stage III and IV gastric cancer patients was significantly lower than that in the stage I and II patients,and the expression of miR-200a in patients with the lymph node metastasis positive group,vascular tumor thrombus positive group and tumor poorly differentiated group was significantly lower than that in the negative group,and the difference was statistically significant(P＜0.05).After knockdown of lncRNA-ATB in BGC-823 cells,the expression of β-catenin and vimentin was decreased and the expression of E-cadherin was increased,and the differences were statistically significant(P＜0.05). Conclusion lncRNA-ATB can affect the progression of gastric cancer by binding to miR-200a,affecting the expression of β-catenin and promoting the process of EMT.

Key words: Gastric cancer, lncRNA-ATB, Epithelial-mesenchymal transition

CLC Number:

R735.2

ZHI Lianghui, LIU Wei, LI Wei, ZHEN Sihu, JIAO Xilin. Mechanism of lncRNA-ATB mediated epithelial mesenchymal transition in the progression of gastric cancer[J]. Journal of Practical Oncology, 2022, 36(3): 208-213.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2022.03.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2022/V36/I3/208

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Mechanism of lncRNA-ATB mediated epithelial mesenchymal transition in the progression of gastric cancer

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