长链非编码RNA EPB41L4A-AS2对乳腺癌的预后研究

doi:10.11904/j.issn.1002-3070.2017.03.003

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (3): 205-210.doi: 10.11904/j.issn.1002-3070.2017.03.003

长链非编码RNA EPB41L4A-AS2对乳腺癌的预后研究

陈芊霖，庞达

哈尔滨医科大学(哈尔滨 150081)

收稿日期:2017-01-06 出版日期:2017-06-28 发布日期:2017-07-04
通讯作者: 庞达,E-mail:pangdasir@163.com
作者简介:陈芊霖,女,(1991-),硕士研究生,从事乳腺肿瘤学方向的研究

Long noncoding RNA EPB41L4A-AS2 associated with the prognosis of tumors

CHEN Qianlin,PANG Da

Harbin Medical University,Harbin 150081,China

Received:2017-01-06 Online:2017-06-28 Published:2017-07-04

摘要/Abstract

摘要： 目的通过检测乳腺癌组织中EPB41L4A-AS2的表达,评价其临床病理特征与乳腺癌患者预后之间的关系。方法利用癌症基因组Meta分析、TCGA和GEO数据集,评价EPB41L4A-AS2的表达量与乳腺癌的临床组织特征之间的关系。利用Western blot实验,在乳腺癌细胞水平验证EPB41L4A-AS2与凋亡通路的关联性。结果在Meta分析、TCGA和基因表达库(GEO)数据集的结果中发现,乳腺癌组织中EPB41L4A-AS2低表达,并且与不良的临床病理特征呈正相关。而在乳腺癌细胞系中的实验验证了EPB41L4A-AS2与细胞经典凋亡通路相关联。在GEO中进行Meta分析的预后研究中,发现EPB41L4A-AS2的高表达与良好的疾病预后有关。结论 EPB41L4A-AS2抑制了肿瘤的形成,对乳腺癌的临床预后分析有较高价值。

关键词: 长链非编码RNA, 乳腺癌, EPB41L4A-AS2

Abstract: Objective The relationship between the clinicopathological features and the prognosis of breast cancer patients was evaluated by the expression of EPB41L4A-AS2 in breast cancer tissues.Methods The relationship between the expression of EPB41L4A-AS2 and the clinical features of breast cancer was evaluated by using the genome meta analysis,TCGA and Gene Expression Library(GEO)datasets.The correlation between EPB41L4A-AS2 and apoptotic pathway was verified by Western blotting.Results The results from Meta-analysis,TCGA and GEO datasets showed that EPB41L4A-AS2 was low in breast cancer tissues and was positively correlated with poor clinical and pathological features.EPB41L4A-AS2 was confirmed an association with the classical apoptosis pathway in breast cancer cell lines.In the meta-analysis of GEO,we found the high expression of EPB41L4A-AS2 with good prognosis.Conclusion EPB41L4A-AS2 inhibits tumor formation and has a high value in clinical prognosis of breast cancer.

Key words: Long non-coding RNA, Breast cancer, EPB41L4A-AS2

中图分类号:

R737.3

陈芊霖，庞达. 长链非编码RNA EPB41L4A-AS2对乳腺癌的预后研究[J]. 实用肿瘤学杂志, 2017, 31(3): 205-210.

CHEN Qianlin,PANG Da. Long noncoding RNA EPB41L4A-AS2 associated with the prognosis of tumors[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 205-210.

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长链非编码RNA EPB41L4A-AS2对乳腺癌的预后研究

Long noncoding RNA EPB41L4A-AS2 associated with the prognosis of tumors

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