TMIGD1在结肠癌中的表达及对结肠癌细胞增殖和侵袭的影响

doi:10.11904/j.issn.1002-3070.2021.03.008

实用肿瘤学杂志 ›› 2021, Vol. 35 ›› Issue (3): 237-242.doi: 10.11904/j.issn.1002-3070.2021.03.008

TMIGD1在结肠癌中的表达及对结肠癌细胞增殖和侵袭的影响

吴云桦¹, 谭敏¹, 王子君¹, 孙学军¹, 李凡妮²

1.西安交通大学第一附属医院普通外科(西安 710061);
2.西安交通大学第一附属医院人才高地科研平台

收稿日期:2020-10-17 修回日期:2021-03-16 出版日期:2021-06-28 发布日期:2021-06-22
通讯作者: 李凡妮,E-mail:fannycpu@163.com
作者简介:吴云桦,男,(1990-),博士,住院医师,从事消化道肿瘤防治相关的研究。
基金资助:
陕西省重点研发计划(编号:2019KW-067)

The expression of TMIGD1 in colon cancer and its effect on the proliferation and invasion of colon cancer cells

WU Yunhua¹, TAN Min¹, WANG Zijun¹, SUN Xuejun¹, LI Fanni²

1. The First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,China;
2. Talent Highland Scientific Research Platform of The First Affiliated Hospital of Xi′an Jiaotong University

Received:2020-10-17 Revised:2021-03-16 Online:2021-06-28 Published:2021-06-22

摘要/Abstract

摘要： 目的检测TMIGD1在结肠癌及其相应的癌旁正常结肠上皮组织中的表达情况及过表达TMIGD1对结肠癌细胞生物学行为的影响,并探讨潜在分子机制。方法通过生物信息学数据库和免疫组织化学染色检测TMIGD1在结肠癌与癌旁正常组织中的表达情况,并分析其与不同临床病理因素之间的关系。对结肠癌细胞系SW480通过慢病毒包装系统使其稳定过表达TMIGD1,采用MTT、平板克隆形成实验、细胞周期试剂盒检测TMIGD1对结肠癌细胞增殖、克隆形成及细胞周期的影响,并通过Western blot检测细胞周期关键蛋白的表达。采用Transwell实验检测细胞迁移和侵袭情况,并检测EMT相关蛋白的表达和细胞粘附情况。结果生物信息学分析和免疫组织化学染色结果均提示TMIGD1在结肠癌组织中低表达(P＜0.05),TMIGD1的阴性表达与结肠癌的淋巴结转移和TNM分期密切相关(P＜0.05)。MTT实验、平板克隆形成实验、细胞周期检测结果表明过表达TMIGD1能够抑制结肠癌细胞SW480的增殖和克隆形成过程,提高G0/G1期的细胞数目,并且抑制Cyclin D1的表达,同时促进p21表达(P＜0.05)。TMIGD1对EMT蛋白没有影响,但稳定过表达TMIGD1后结肠癌细胞的迁移和侵袭能力明显降低(P＜0.05),而粘附能力明显增强。结论 TMIGD1在结肠癌中低表达,过表达TMIGD1能够抑制结肠癌细胞的增殖和迁移侵袭。

关键词: 结肠癌, TMIGD1, 细胞增殖, 细胞侵袭

Abstract: Objective The aims of this study were to detect the expression of TMIGD1 in colon cancer tissues and its corresponding adjacent colonic epithelial tissues,the effect of overexpression of TMIGD1 on the biological behaviors of colon cancer cells and to explore the potential molecular mechanisms.Methods The bioinformatics database and immunohistochemical staining were used to detect the expression of TMIGD1 in colon cancer and its corresponding adjacent colon tissues,and analyze its relationship with different clinicopathological factors.The colon cancer SW480 cells were stably overexpressed TMIGD1 through the lentiviral packaging system.MTT,clone formation experiment,cell cycle kit and Western blot were used to detect the effect of TMIGD1 on proliferation,clone formation,cell cycle and the expression of key proteins in the cell cycle of colon cancer cells.The Western blot was used to detect the expression of EMT-related proteins in SW480 cells,and Transwell experiment was used to detect cell migration and invasion.Results The results of bioinformatics analysis and immunohistochemical staining showed that TMIGD1 was low expressed in colon cancer tissues(P＜0.05).The negative expression of TMIGD1 was closely related to the lymph node metastasis and TNM staging of colon cancer(P＜0.05).The results of MTT,clone formation experiments and cell cycle analyses showed that overexpression of TMIGD1 could inhibit the proliferation and colony formation,and increase the number of cell cycle at the G0/G1 phase,and inhibit the expression of Cyclin D1,while promoting the expression of p21 in colon cancer SW480 cells(P＜0.05).TMIGD1 had no effect on EMT protein,but after stably overexpressing TMIGD1,the migration and invasion abilities of colon cancer cells were significantly reduced,while the adhesion ability was significantly enhanced(P＜0.05).Conclusion TMIGD1 is low expressed in colon cancer.Overexpression of TMIGD1 can inhibit the proliferation,migration and invasion of colon cancer cells.

Key words: Colon cancer, TMIGD1, Cell proliferation, Cell invasion

中图分类号:

R735.3

吴云桦, 谭敏, 王子君, 孙学军, 李凡妮. TMIGD1在结肠癌中的表达及对结肠癌细胞增殖和侵袭的影响[J]. 实用肿瘤学杂志, 2021, 35(3): 237-242.

WU Yunhua, TAN Min, WANG Zijun, SUN Xuejun, LI Fanni. The expression of TMIGD1 in colon cancer and its effect on the proliferation and invasion of colon cancer cells[J]. Journal of Practical Oncology, 2021, 35(3): 237-242.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2021.03.008

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2021/V35/I3/237

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TMIGD1在结肠癌中的表达及对结肠癌细胞增殖和侵袭的影响

The expression of TMIGD1 in colon cancer and its effect on the proliferation and invasion of colon cancer cells

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