长链非编码RNA AFAP1-AS1通过PTEN/p-AKT信号通路调控结直肠癌细胞增殖的分子机制研究

doi:10.11904/j.issn.1002-3070.2020.01.003

Abstract

Abstract: Objective The aim of this study was to explore the molecular mechanism of long non-coding RNA(LncRNA)AFAP1-AS1 regulating the proliferation of colorectal cancer(CRC)cells.Methods Fecal specimens from 38 normal people and 38 CRC patients diagnosed and treated in the department of digestive medicine and gastrointestinal surgery of Shenzhen Second People′s Hospital from July 2014 to June 2018 were collected to detect the expression of lncRNA AFAP1-AS1 by Real time-PCR.At the same time,the expression of lncRNA AFAP1-AS1 was detected in human normal colon epithelial NCM460 cells,human colorectal cancer SW620 and HCT116 cells.Targeting lncRNA AFAP1-AS1 siRNA(si-AFAP1-AS1)was transfected into SW620 and HCT116 cells to inhibit the expression of AFAP1-AS1.MTT assay was used to examine the effect of si-AFAP1-AS1 on cell proliferation of CRC cells.The levels of cleaved-caspase-3,Bcl-2,Bax,p-AKT,total AKT,and PTEN were detected by Western blot.Results Compared with the normal control group,the expression of AFAP1-AS1 in feces of CRC patients significantly increased(P＜0.01);Compared with NCM460 cells,the expression of AFAP1-AS1 in SW620 and HCT116 cells also significantly increased(P＜0.01);si-AFAP1-AS1 significantly suppressed the cell viability of SW620 and HCT116 cells(P＜0.01).Compared with NCM460 cells,si-AFAP1-AS1 up-regulated the levels of cleaved-caspase-3 and pro-apoptotic protein Bax(P＜0.05),and down-regulated the level of anti-apoptotic protein Bcl-2 in SW620 and HCT116 cells(P＜0.001).In addition,si-AFAP1-AS1 significantly decreased the level of p-AKT protein and increased the expression of PTEN in CRC cells(P＜0.01).Conclusion The difference in the expression of lncRNA AFAP1-AS1 in the stool of normal and CRC patients is beneficial to the early diagnosis for CRC,the expression of lncRNA AFAP1-AS1 is up-regulated in CRC cells regulated by the PTEN/p-AKT signaling pathway.Therefore,lncRNA AFAP1-AS1 is expected to become a molecular target for early diagnosis of colorectal cancer.

Key words: AFAP1-AS1, Colorectal cancer, Apoptosis, PTEN/p-AKT signaling pathway

CLC Number:

R735.34

ZENG Li, ZHUANG Shutong, DING Shihua, CHEN Chong, JIAO Lu, SUN Dayong. Molecular mechanisms of long non-coding RNA AFAP1-AS1 regulating the proliferation of colorectal cancer cells by PTEN/p-AKT signaling pathway[J]. Journal of Practical Oncology, 2020, 34(1): 11-16.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.01.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I1/11

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Molecular mechanisms of long non-coding RNA AFAP1-AS1 regulating the proliferation of colorectal cancer cells by PTEN/p-AKT signaling pathway

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