Abstract: Objective To analyze the correlation between Bmi-1,Ki-67 and Bcl-2 expression in colorectal cancer and to explore the correlation and significance with clinicopathological features of colorectal cancer.Methods Immunohistochemistry staining was used to display the expression of Bmi-1,Ki-67 and Bcl-2 in 84 cases of colorectal cancer,84 cases of matched normal colorectal mucosa and 21 cases of precancerous lesion.Results The positive rates of Bmi-1 expression in colorectal cancer(73.81%)and intraepithelial neoplasia(95.23%)were significantly higher than those in normal colorectal mucosa membrane(59.52%),P＜0.05.There was a positive relationship between Bmi-1 and Bcl-2 expression in 84 cases of colorectal cancer(r=0.358,P＜0.001).Conclusion The abnormality of Bmi-1 expression in colorectal cancer might be involved in cell proliferation,apoptosis and cancerization.Bmi-1 was closely correlated with Bcl-2 in colorectal cancer,suggesting that Bmi-1 might coordinate with Bcl-2 to induce anti-apoptosis effect,whereas the suppressing apoptosis pathways were different.