NFATc1对人肺腺癌细胞增殖、凋亡和侵袭能力的影响

doi:10.11904/j.issn.1002-3070.2021.05.003

Abstract

Abstract: Objective The aim of this study was to investigate the effects of NFATc1 expression on the proliferation,apoptosis,migration and invasion of lung adenocarcinoma NCI-H1299 cells,and its specific biological mechanisms. Methods Real time PCR was used to detect the relative levels of NFATc1 in three lung adenocarcinoma cell lines,and the NCI-H1299 cell line with stable knockdown of NFATc1 was constructed using lentivirus vectors.A MTT assay was used to detect the effect of NFATc1 on cell proliferation,the cell colony assay was used to detect the effect of NFATc1 on clone formation,the Transwell assay was used to detect the effect of NFATc1 on cell migration and invasion,and flow cytometry was used to detect the effect of NFATc1 on cell cycle and apoptosis.Western blot was used to detect the effects of NFATc1 on apoptosis,EMT-related proteins and MAPK signaling pathways protein expression. Results The expression of NFATc1 in lung adenocarcinoma NCI-H1299 cells was relatively high.Silencing NFATc1 expression could significantly inhibit cell proliferation(P＜0.05),clone formation(P＜0.05),migration(P＜0.05)and invasion(P＜0.05),the cell cycle arrested at the G1 phase(P＜0.05),and promote apoptosis(P＜0.05).The expression of Bax,cleaved caspase-3 and E-Cadherin protein increased(P＜0.05),and CDK4,c-Myc,ERK,p-ERK and N-Cadherin protein expression decreased(P＜0.05). Conclusion Inhibition of NFATc1 expression in human lung adenocarcinoma NCI-H1299 cells can significantly inhibit cell proliferation,arresting cell cycle,migration and invasion,and promote cell apoptosis.The mechanism may be related to the inhibition of MAPK signaling pathway.

Key words: NFATc1, Lung adenocarcinoma, Molecular mechanism

CLC Number:

R735.3

REN Fenghai, ZHU Kaibin, WANG Yanbo, ZHOU Fucheng, PANG Sainan, CHEN Lantao. Effects of NFATc1 on the proliferation,apoptosis and invasion of human lung adenocarcinoma cells[J]. Journal of Practical Oncology, 2021, 35(5): 402-407.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2021.05.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2021/V35/I5/402

References

1 Jauliac S,López-Rodriguez C,Shaw L,et al.The role of nfat transcription factors in integrin-mediated carcinoma invasion[J].Nature Cell Biology,2002,4(7):540-544.
2 Li L,Duan Z,Yu J,et al.Nfatc1 regulates cell proliferation,migration,and invasion of ovarian cancer skov3 cells in vitro and in vivo[J].Oncology Reports,2016,36(2):918-928.
3 Baumgart S,Chen N,Siveke J,et al.Inflammation-induced nfatc1-stat3 transcription complex promotes pancreatic cancer initiation by krasg12d[J].Cancer Discovery,2014,4(6):688-701.
4 Xu W,Gu J,Ren Q,et al.Nfatc1 promotes cell growth and tumorigenesis in ovarian cancer up-regulating c-myc through erk1/2/p38 mapk signal pathway[J].Tumour biology,2016,37(4):4493-4500.
5 文洪梅,陈杨,石青萍,等.2016年云南省肿瘤登记地区恶性肿瘤的发病和死亡分析[J].实用肿瘤学杂志 2020,34(6):485-490.
6 Miller KD,Nogueira L,Mariotto AB,et al. Cancer treatment and survivorship statistics,2019[J].CA Cancer J Clin,2019,69(5):363-385.
7 Herbst R,Morgensztern D,Boshoff C.The biology and management of non-small cell lung cancer[J].Nature,2018,553(7689):446-454.
8 Kawahara T,Kashiwagi E,Ide H,et al.The role of nfatc1 in prostate cancer progression:Cyclosporine a and tacrolimus inhibit cell proliferation,migration,and invasion[J].The Prostate,2015,75(6):573-584.
9 Buchholz M,Schatz A,Wagner M,et al.Overexpression of c-myc in pancreatic cancer caused by ectopic activation of nfatc1 and the ca2+/calcineurin signaling pathway[J].EMBO J,2006,25(15):3714-3724.
10 Oikawa T,Nakamura A,Onishi N,et al.Acquired expression of nfatc1 downregulates e-cadherin and promotes cancer cell invasion[J].Cancer Res,2013,73(16):5100-5109.
11 Kawahara T,Kashiwagi E,Li Y,et al.Cyclosporine a and tacrolimus inhibit urothelial tumorigenesis[J].Mol Carcinog,2016,55(2):161-169.
12 Kawahara T,Kashiwagi E,Ide H,et al.Cyclosporine a and tacrolimus inhibit bladder cancer growth through down-regulation of nfatc1[J].Oncotarget,2015,6(3):1582-1593.
13 Wang S,Kang X,Cao S,et al.Calcineurin/nfatc1 pathway contributes to cell proliferation in hepatocellular carcinoma[J].Dig Dis Sci,2012,57(12):3184-3188.
14 Iñiguez M,Martinez-Martinez S,Punzón C,et al.An essential role of the nuclear factor of activated t cells in the regulation of the expression of the cyclooxygenase-2 gene in human t lymphocytes[J].J Biol Chem,2000,275(31):23627-23635.
15 Liu Y,Liang T,Qiu X,et al.Down-regulation of nfatc1 suppresses proliferation,migration,invasion,and warburg effect in prostate cancer cells[J].Med Sci Monit,2019,25:1572-1581.
16 Sun Y,Liu WZ,Liu T,et al.Signaling pathway of mapk/erk in cell proliferation,differentiation,migration,senescence and apoptosis[J].J Recept Signal Transduct Res,2015,35(6):600-604.
17 Mondal A,Biswas R,Rhee YH,et al.Sulforaphene promotes bax/bcl2,mapk-dependent human gastric cancer ags cells apoptosis and inhibits migration via egfr,p-erk1/2 down-regulation[J].Gen Physiol Biophys,2016,35(1):25-34.
18 Lin H,Hao Y,Wan X,et al.Baicalein inhibits cell development,metastasis and emt and induces apoptosis by regulating erk signaling pathway in osteosarcoma[J].J Recept Signal Transduct Res,2020,40(1):49-57.
19 Yang J,Zhang JN,Chen WL,et al.Effects of aqp5 gene silencing on proliferation,migration and apoptosis of human glioma cells through regulating egfr/erk/p38 mapk signaling pathway[J].Oncotarget,2017,8(24):38444-38455.

[1]	WANG Junmei, PAN Huiqing, LI Jingyi, JIA Di, ZHAO Weiming, LV Changlian. The expression and clinical significance of MT2-MMP at the stage II of lung adenocarcinoma [J]. Journal of Practical Oncology, 2021, 35(3): 243-247.
[2]	HAN Jing, PU Yan, LIU Huibin. Effects of SKA1 on cell proliferation and apoptosis of renal cell carcinoma and its molecular mechanism [J]. Journal of Practical Oncology, 2020, 34(5): 404-411.
[3]	ZHANG Mengzhe, KANG Xiangdong. Research progress on the relationship between Krüpple-like transcription factor 4 and the development of colorectal cancer [J]. Journal of Practical Oncology, 2020, 34(5): 456-459.
[4]	CHEN Lantao, YAN We, LIU Jinglei, YAO Lei. Effect of tetrandrine on lung cancer H1299 cells and A549 cells and its molecular mechanism [J]. Journal of Practical Oncology, 2020, 34(2): 126-132.
[5]	LIU Guangxia, ZHAO Lianchun, CHEN Fang, JIA Qian, GAO Wei, HOU Zhan, LU Yamin. Effect and mechanism of ZCCHC12 on malignant transformation of thyroid cells [J]. Journal of Practical Oncology, 2020, 34(2): 133-138.
[6]	LI Yafei, ZHANG Xiao, WANG Xuefei, WENG Mingjiao, WANG Tianzhen. Effect of RNA-binding protein Lin28 on the development of malignant tumors and its molecular mechanism [J]. Journal of Practical Oncology, 2019, 33(5): 456-460.
[7]	MA Jianli, ZHANG Minghui, JI Hongfei, XU Xiangying. Clinical significance of combined detection of PD-L1 protein and CD8⁺TILs in resected lung adenocarcinoma [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(1): 40-46.
[8]	BIAN Xiusen, LI Guang, GUAN Xinyu, ZHANG Yi, DI Chang, MA Can. Molecular mechanism of UHRF1 inhibiting proliferation of lung adenocarcinoma cells by regulating autophagy [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 498-502.
[9]	KAN Jun, ZHOU Daihan, ZHANG Enxin. Research progress in tumor with cardiovascular disease [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 558-561.
[10]	FAN Ningning, GENG Jingshu. The expression and role of LncRNA MEG3 in human glioma cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(3): 219-223.
[11]	YUAN Meng,LI Xiaoli. Research progress of the relationship between TRAP1 and malignant tumor [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(4): 371-375.
[12]	LI Zhiwei,WANG Fengyun,ZHANG Qianqian,LI Dingnan,ZHANG Chunhui,YAN Feihu,ZHANG Yanqiao. STAT3 promote lung adenocarcinoma EMT by up-regulating Twist expressing [J]. PRACTICAL ONCOLOGY JOURNAL, 2014, 28(4): 310-314.
[13]	LIU Jinglei, JIANG Ji, ZHANG Mingyan, WANG Ying, LIU Yongrui, WANG Yan. Studies of TMP on restraining proliferation and reversing multidrug resistance in human lung adenocarcinoma cell line [J]. PRACTICAL ONCOLOGY JOURNAL, 2013, 27(4): 303-307.
[14]	YAO Zhifeng, YAO Jianxin, XU Guoping, LIU Yongbiao. Radiosensitization of Cetuximab on pulmonary adenocarcinoma A549 cells line [J]. PRACTICAL ONCOLOGY JOURNAL, 2013, 27(1): 11-16.

Effects of NFATc1 on the proliferation,apoptosis and invasion of human lung adenocarcinoma cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments