Abstract: Objective To observe the effects of Ursolic acid(UA)on adhesion,invasion and migration of HO-8910PM cell line in vitro,and discuss its possible mechanism.Methods Effect of UA on adhesion of HO-8910PM cells were determined with MTT assay,the effects of UA on invasion and migration of HO-8910PM cells were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane.The exprssion of MMP-2 mRNA,MMP-9 mRNA was measured by RT-PCR in HO-8910PM cells,the protein expression of MMP-2,MMP-9 was analyzed by Western blot.Results In the cell-Matrigel adhesion experiment,inhibition rate of UA groups at different concentrations of 10,20,30μmol/L for 24h were 44.03%,46.62% and 55.11% respectively which were significantly higher than those of control groups(P＜0.05),after 24 hours,the ability of the different concentrations of UA treated HO-8910PM cells to invade the reconstituted basement membrane decreased significantly(P＜0.01).UA can also inhibit HO-8910PM cells migration(P＜0.05).RT-PCR and Western blot results showed decreased expression of MMP-2,MMP-9 in HO-8910PM cells treated with(10,20,30μmol/L)UA for 24 hours.Conclusion The inhibition of invasion ability of HO-8910PM cells by UA may be attributed to decreases of the expression of MMP-2,MMP-9.