Abstract: Objective To observe the growth inhibitory effect of oxaliplatin(L-OHP)and(7-hydroxystaurosporine,UCN-01)on HCT116 colon cancer cell line in single or combination treatment,and to investigate its mechanisms.Method The growth inhibitory effect was evaluated by MTT assay in single agent or combination treatment.Apoptosis was investigated by flow cytometry and Western blotting,and mitotic catastrophy was determined by HE staining.Results Combination treatment of L-OHP in low concentration and UCN-01 has synergistic effect on killing HCT116 colon cancer cell line,which was more effective than single treatment.AnnerxinV positive cell rate showed no difference between combination treatment and UCN-01 alone.Cellular morphology of HCT116 cell was changed in combination treatment,and megakaryocytes and polykaryocytes in combination treatment were increased by approximately 10% compared with the single treatment.Conclusion Combinition treatment of L-OHP and UCN-01 has synergistic inhibitory effect on HCT116 cell proliferation through inducing cell apoptosis and mitotic catastrophy.