Abstract: Objective To observe the influence of SPS on the proliferation of PBMC and CD8⁺ cells in vitro.Methods PBMC extracted from healthy donors with ficoll-hypaque density gradient centrifugation were incubated with different doses of SPS in vitro.The proliferative activity of PBMC was tested by 3H-TdR assay and the proliferative activity of CD8⁺ cells was tested by fluorescence-activated cell sorter(FACS).Results SPS can accelerate the proliferation of PBMC with dose-dependent manner and was obvious at 1.25g·L^-1 and 0.625g·L^-1 dose group(P＜0.05);SPS can obviously promote the proliferation of CD8⁺ cells(P＜0.05).Conclusion SPS can promote the proliferation of PBMC and CD8⁺ cells,enhance the immune function of the organism.

Key words: Safflower polysaccharide(SPS), Peripheral blood mononuclear cells(PBMC), Proliferation, Fluorescence-activated cell sorter(FACS)