Based on the analysis of core,thin section and logging data,basin-scale microbial carbonate with stable distribution developed in the lower part of the Ma55 sub-member of the Ordovician Majiagou Formation in Ordos Basin. The rock types include argillaceous limestone,limestone,stromatolite,thrombolite,bound sand-clastic limestone,karst breccias. It contains various shaped stromatolite and thrombolite,such as laminar,wavy and columnar stromatolite,laminar,nodular and arborescent thrombolite. Vertically,four typical sedimentary sequences closely related to microbialites are identified,indicating relative sea-level fluctuations. The division and comparative analysis of the small layers with high-frequency cycles suggest that the microbialites are stably distributed laterally,and have basin-scale distribution. Based on the logging template established by core-log calibration,the lithology of 404 wells in the whole basin were interpreted.The result shows that cumulative thickness of microbialites range from 3 to 7m,and the thickness shows a decreasing trend from west to east.Furthermore,the continuous distribution area of microbialites can exceed 2.5×105km2in area,demonstrating that the basin-scale microbialites developed in a relatively shorter time.Analysis of sedimentary sequence in the early depositional stage of Ma55 sub-member indicates that the relative sea level first slowly rises and then falls in a single cycle,while it shows an oscillating and slowly rising trend in a multi-cycle combination.Further research suggests that the relatively stable paleo-tectonic environment coupled with the filling of early evaporite provides relatively flat paleo-geomorphic conditions.Under the background of continuous slow transgression in the depositional stage of Ma55 sub-member,the bottom shape condition of environmental homogeneity is the fundamental reason for the development of large-scale microbial carbonate in shallow water.This paper reports the development and distribution of basin-scale microbialites in the depositional stage of Ma55 sub-member,which not only enriches the research of microbialites in the Majiagou Formation,but also helps improve the understanding of development mechanism of large-scale microbialites in carbonate platforms.