关键词: 微小RNA, miR-222, PTEN, 肝细胞肝癌

Abstract:

Objective To detect the changes of cell invasion and proliferation in hepatocellular carcinoma cell line HepG2 through the upregulation of miR-222.Methods The expression of miR-222 was up-regulated by liposomal transfection.The expression of PTEN which is the targeting protein of miR-222 was detected by using Western Blot assay.The changes of proliferative capacity and invasive ability in HepG2 cells after the upregulation of miR-222 were detected through MTT assay and Transwell assay.Results In Western Blot experiments,the expression of PTEN in transfection groups were lower than that in negative control groups and blank control groups.In MTT assay,the OD values in transfected group were higher than that in negative control group and blank control group(P＜0.05).In transwell experiments,the number of penetrating cells in transfected group were higher than that in negative control group and blank control group(P＜0.05).Conclusion Our Results suggest that PTEN is significantly down regulated by the upregulation of miR-222.The proliferative capacity and invasive ability are raised in hepatocellular carcinoma cell line HepG2 through the upregulation of miR-222.

Key words: MicroRNA, miR-222, PTEN, Hepatocellular carcinoma