甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响

doi:10.11904/j.issn.1002-3070.2017.05.002

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (5): 390-395.doi: 10.11904/j.issn.1002-3070.2017.05.002

甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响

王吉锡, 邓伟生, 王凤儒, 史之茂, 孟丹, 徐强, 张玉瑶

黑龙江中医药大学(哈尔滨 150040)

收稿日期:2017-02-09 出版日期:2017-10-28 发布日期:2017-10-24
通讯作者: 王凤儒,E-mail:wangfengrurr@163.com
作者简介:王吉锡,男,(1979-),博士,副教授,从事中草药抗肿瘤作用的研究
基金资助:
黑龙江省教育厅科学技术研究项目(12541764)

Active part of Glycyrrhiza extract induces apoptosis in human cervical cancer HeLa cells and its related protein caspase-3 and caspase-9 expression

WANG Jixi, DENG Weisheng, WANG Fengru, SHI Zhimao, MENG Dan, XU Qiang, ZHANG Yuyao

Heilongjiang University of Chinese Medicine,Harbin 150040,China

Received:2017-02-09 Online:2017-10-28 Published:2017-10-24

摘要/Abstract

摘要： 目的研究甘草提取物的有效部位(GL)体外诱导HeLa细胞凋亡情况及对Caspase家族蛋白表达的调控,进一步阐明GL的抑癌机制。方法将25 μg/mL的GL作用于HeLa细胞24 h后,采取MTT比色法,吖啶橙/溴乙锭荧光双染法,透射电子显微镜法,观察GL诱导HeLa细胞凋亡情况,并采用Western blot法检测Caspase-3、Caspase-9蛋白表达水平的变化。结果 MTT结果显示,GL可以有效抑制HeLa细胞的增殖,呈剂量-时间相关性。AO/EB双染色及透射电镜观察发现GL作用HeLa细胞24 h后,可见大量早期凋亡细胞。Western blot测定结果显示,药物组Pro-caspase-9蛋白表达量与对照组比较差异具有统计学意义(P＜0.05);Cleaved-caspase-3蛋白表达量高于对照组。结论 GL能够促进Caspase-3、Caspase-9酶原活化,具有体外诱导HeLa细胞凋亡的作用。

关键词: 甘草, 抗肿瘤作用, 细胞凋亡, HeLa细胞, 凋亡蛋白

Abstract: Objective The aim of this study was to determine whether active part of Glycyrrhiza extract(GL)induced apoptosis in Hela cells and its inhibitory mechanism.Methods HeLa cells were treated with 25 g/mL of GL for 24hs.Cell viability and apoptosis in HeLa cells were determined by MTT,AO/EB fluorescent double staining,transmission electron microscope(TEM),and Western blot.Results The MTT results showed that GL significantly inhibited the proliferation of HeLa cells in a dose-response.After treatment for 24 hrs,large number of early apoptotitc cell were observed using AO/EB fluorescent double staining and TEM.The expression of Pro-caspase-9 and Cleaved-caspase-3 protein was higher in GL-treated cells them those of the control cells(P＜0.05).Conclusion GL can activate Caspase-3 and Caspase-9 genes to induce apoptosis in HeLa cells.

Key words: Licorice, Anti-tumor effect, Apoptosis, HeLa cells, Apoptosis protein

中图分类号:

R737.33

王吉锡, 邓伟生, 王凤儒, 史之茂, 孟丹, 徐强, 张玉瑶. 甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响[J]. 实用肿瘤学杂志, 2017, 31(5): 390-395.

WANG Jixi, DENG Weisheng, WANG Fengru, SHI Zhimao, MENG Dan, XU Qiang, ZHANG Yuyao. Active part of Glycyrrhiza extract induces apoptosis in human cervical cancer HeLa cells and its related protein caspase-3 and caspase-9 expression[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(5): 390-395.

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甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响

Active part of Glycyrrhiza extract induces apoptosis in human cervical cancer HeLa cells and its related protein caspase-3 and caspase-9 expression

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