苏林酸在NF-κB对人乳腺癌TNF-α诱导细胞凋亡增敏中的相关作用

doi:10.11904/j.issn.1002-3070.2017.02.002

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (2): 102-106.doi: 10.11904/j.issn.1002-3070.2017.02.002

苏林酸在NF-κB对人乳腺癌TNF-α诱导细胞凋亡增敏中的相关作用

张绪良¹, 胡亚华¹, 黄丹青²

1.鄂东医疗集团黄石市中心医院普爱院区乳腺肿瘤外1科(黄石 435000);
2.鄂东医疗集团黄石市中心医院超声科

收稿日期:2016-10-22 出版日期:2017-04-20 发布日期:2017-05-04
通讯作者: 黄丹青,E-mail:huangdanq33@163.com
作者简介:张绪良,男,(1980-),硕士,主治医师,从事乳腺及甲状腺肿瘤方面的研究
基金资助:
黄石市科学技术局项目(2015b016-13)

Sulindac enhances sensitization effect of NF-κB on apoptosis induced by TNF-α in human breast cancer

ZHANG Xuliang¹,HU Yahua¹,HUANG Danqing²

1.Department of Breast Cancer Surgery,Edong Healthcare Huangshi Central Hospital,Puai District,Huangshi 435000,China;
2.Department of Ultrasonography,Edong Healthcare Huangshi Central Hospital

Received:2016-10-22 Online:2017-04-20 Published:2017-05-04

摘要/Abstract

摘要： 目的探讨苏林酸在NF-κB对人乳腺癌TNF-α诱导细胞凋亡增敏中的相关作用。方法取对数生长期的人乳腺癌细胞系MCF-7,加入苏林酸,使其终浓度分别为0.5mmol/L和1.0mmol/L,并设不加苏林酸的对照组进行培养;苏林酸处理48h后进行MTT、流式细胞实验和Western blot法检测,分析苏林酸对肿瘤细胞生长的作用与相关机制。结果 0.5mmol/L和1.0mmol/L苏林酸分别刺激MCF-7细胞48h后,对应的MCF-7细胞的增殖抑制率为(29.17±1.23)%、(38.15±1.51)%,对照组为(1.15±0.02)%(P＜0.05)。与对照组相比,0.5mmol/L和1.0mmol/L苏林酸作用后均可导致滞留在G₀~G₁期的MCF-7细胞显著增加(P＜0.05);0.5mmol/L和1.0mmol/L苏林酸作用后MCF-7细胞的凋亡率明显增加(P＜0.05);0.5mmol/L和1.0mmol/L苏林酸作用后TNF-α的相对表达量分别为2.09±0.67、1.18±0.09,明显低于对照组的7.42±0.56(P＜0.05)。结论苏林酸具有一定抗人乳腺癌细胞生长的作用,能促使细胞周期G₀~G₁期延长,提高细胞凋亡增敏作用,其作用机制可能与抑制TNF-α活性有关。

关键词: 苏林酸, NF-κB, TNF-α, 细胞凋亡, 增敏作用

Abstract: Objective The objective of this study was to investigate the role of sulindac in sensitization effects of NF-κB on apoptosis induced by TNF-α in human breast cancer.Methods The human breast cancer MCF-7 cell line was added sulindal in the logarithmic growth phase and the final concentrations of sulindac were 0.5 and 1.0mmol/L.The cells in control group was cultured without adding succinic acid.After sulindac treatment for 48h,flow cytometry,MTT and Western blotting were used to analyze the effect and mechanism of cell growth in MCF-7 cells.Results The inhibitory rate of cell proliferation was(29.17±1.23)% and(38.15±1.51)% in MCF-7 cells treated with 0.5 and 1.0mmol/L of Sulindac for 48h,respectively,when compared to the control group(1.15 ± 0.02)%(P＜0.05).Compared with the control group,0.5 and 1.0mmol/L of sulindac were significantly increased the G₀/G₁ phase in MCF-7 cells(P＜0.05).The apoptosis rate of sulindac in MCF-7 cells was significantly higher than that in the control group(P＜0.05).The expression levels of TNF-α were(2.09±0.67)% and(1.18±0.09)% in the concentrations of 0.5 and 1.0mmol/L sulindac,respectively,in MCF-7 cells when compared to the control group(7.42±0.56)%.Conclusion Sulindac has a certain effect on the growth of human breast cancer cells,which can promote the prolongation of cell cycle at the G₀/G₁ phase and improve sensitization of apoptosis.This mechanism may be related to the inhibition of TNF-α activity.

Key words: Sulindac, NF-κB, TNF-α, Cell apoptosis, Sensitization effect

中图分类号:

R737.9

张绪良, 胡亚华, 黄丹青. 苏林酸在NF-κB对人乳腺癌TNF-α诱导细胞凋亡增敏中的相关作用[J]. 实用肿瘤学杂志, 2017, 31(2): 102-106.

ZHANG Xuliang,HU Yahua,HUANG Danqing. Sulindac enhances sensitization effect of NF-κB on apoptosis induced by TNF-α in human breast cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(2): 102-106.

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苏林酸在NF-κB对人乳腺癌TNF-α诱导细胞凋亡增敏中的相关作用

Sulindac enhances sensitization effect of NF-κB on apoptosis induced by TNF-α in human breast cancer

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