Aurora-A在宫颈癌及癌前病变中的表达以及与HPV感染的相关性研究

doi:10.11904/j.issn.1002-3070.2017.06.003

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (6): 494-499.doi: 10.11904/j.issn.1002-3070.2017.06.003

Aurora-A在宫颈癌及癌前病变中的表达以及与HPV感染的相关性研究

雷蕾¹, 叶庆华¹, 王国增¹, 黄贤莉¹, 吴乾渝²

1.同济大学附属东方医院妇产科(上海 200123)
2.上海市卫生和计划生育委员会

收稿日期:2017-05-18 出版日期:2017-12-28 发布日期:2018-01-02
通讯作者: 吴乾渝,E-mail:wuqianyu369@163.com
作者简介:雷蕾,女,(1980),硕士,主治医师,从事妇科肿瘤的研究。
基金资助:
上海市卫生和计划生育委员会面上项目(201640246)

Expression of Aurora-A in cervical cancer and precancerous lesions,and its correlation with HPV infection

LEI Lei¹, YE Qinghua¹, WANG Guozeng¹, HUANG Xianli¹, WU Qianyu²

1.Department of Obstetrics and Gynecology,Dongfang Hospital,Tongji University,Shanghai 200123,China
2.Shanghai Municipal Health and Family Planning Commission

Received:2017-05-18 Online:2017-12-28 Published:2018-01-02

摘要/Abstract

摘要： 目的分析正常宫颈、不同级别宫颈上皮内瘤变(Cervical intraepithelial neoplasia,CIN)和宫颈鳞状细胞癌(Cervical squamous cell carcinoma,SCC)组织中Aurora-A的表达差异,观察其异常表达对宫颈癌变过程的影响及与人乳头瘤病毒(Human papilloma virus,HPV)感染的相关性,了解Aroura-A在CIN与宫颈癌发病机制中的作用。方法选取已进行宫颈管分泌物高危型HPV检测的宫颈活检或手术标本存档蜡块100例,其中正常宫颈组织20例,CIN1级组织20例、CIN 2级组织20例、CIN 3级组织20例,宫颈癌全部选取的是宫颈鳞状细胞癌组织20例。采用免疫组化方法检测Aurora-A蛋白的表达水平,并分析Aurora-A表达与HR-HPV感染的相关性。结果 Aurora-A在CIN以及宫颈癌中存在高表达(P＜0.05),且其阳性表达率随着宫颈病变程度的加深而增强,两者呈正相关性(r=0.475,P＜0.001);在高级别宫颈上皮内瘤变(CIN2、3)组织中,Aurora-A表达与HPV感染间存在正相关(V=0.591,P＜0.05)。结论 Aurora-A的异常表达可能与宫颈癌的发生发展密切有关,Aurora-A可能作为早期诊断CIN或宫颈癌的重要分子生物学指标,同时也可能是宫颈癌的潜在治疗靶点。

关键词: Aurora-A, 中心体, 人乳头瘤病毒, 宫颈上皮内瘤变, 宫颈癌

Abstract: Objective The objectives of this study were to investigate the expression of Aurora-A protein in cervical cancer and precancerous lesions,and its relationship with human papilloma virus(HPV)infection,and to analyze the role of Aurora-A in the pathogenesis of cervical cancer.Methods One hundred cases of cervical biopsy or surgical resection specimens were collected from high-risk HPV(HR-HPV)test.There were 20 cases of normal cervical tissues,20 cases of CIN grade 1(CIN1),20 cases of CIN grade 2(CIN2),20 cases of CIN grade 3(CIN3),and 20 cases of cervical squamous cell carcinoma.The expression of Aurora-A protein was detected by immunohistochemistry and the correlation between Aurora-A expression and HR-HPV infection was analyzed.Results Aurora-A was highly expressed in cervical intraepithelial neoplasia and cervical cancer(P＜0.05),and its positive expression rate increased with the degree of cervical lesions.There was a positive correlation between Aurora-A expression and cervical cancer(r=0.475,P＜0.001).There was a positive correlation between Aurora-A expression and HR-HPV infection in CIN2 and CIN3(V=0.591,P＜0.05).Conclusion Aurora-A may be associated with the development of cervical cancer.Aurora-A can be used as an important biomarker for the early diagnosis of cervical intraepithelial neoplasia or cervical cancer.It is also a potential therapeutic target for cervical cancer.

Key words: Aurora-A, Centrosome, Human papillomavirus, Cervical intraepithelial neoplasia, Cervical cancer

中图分类号:

R737.3

雷蕾, 叶庆华, 王国增, 黄贤莉, 吴乾渝. Aurora-A在宫颈癌及癌前病变中的表达以及与HPV感染的相关性研究[J]. 实用肿瘤学杂志, 2017, 31(6): 494-499.

LEI Lei, YE Qinghua, WANG Guozeng, HUANG Xianli, WU Qianyu. Expression of Aurora-A in cervical cancer and precancerous lesions,and its correlation with HPV infection[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(6): 494-499.

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Aurora-A在宫颈癌及癌前病变中的表达以及与HPV感染的相关性研究

Expression of Aurora-A in cervical cancer and precancerous lesions,and its correlation with HPV infection

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