长链非编码RNA PSMA3-AS1在人胶质瘤细胞中的表达及作用研究

doi:10.11904/j.issn.1002-3070.2020.03.005

摘要/Abstract

摘要： 目的探究胶质瘤组织中lncRNA PSMA3-AS1的表达情况,以及其对胶质瘤细胞(U251、LN229、T98G和A172)增殖和侵袭能力的影响。方法使用qRT-PCR实验检测35对胶质母细胞瘤组织和癌旁组织中PSMA3-AS1表达;结合TCGA数据库分析PSMA3-AS1表达与胶质瘤恶性程度及患者预后的关系;采用U251和T98G胶质瘤细胞系进行细胞增殖能力和侵袭能力检测;使用Western blot实验检测侵袭相关蛋白MMP-2/9表达水平。结果 TCGA数据库分析提示PSMA3-AS1在胶质瘤组织中呈高表达状态,结合35对胶质瘤组织和细胞系中PSMA3-AS1的表达,提示PSMA3-AS1在胶质瘤中表达明显上调(P＜0.05)。同时发现PSMA3-AS1表达与肿瘤直径有关(P=0.007),而PSMA3-AS1高表达的胶质瘤患者生存期较短(P=0.042)。敲低PSMA3-AS1表达后,胶质瘤细胞内PSMA3-AS1的表达水平及细胞增殖、侵袭能力明显受到抑制,侵袭相关蛋白(MMP-2/9)表达水平明显降低(P＜0.05)。结论 PSMA3-AS1在胶质瘤中呈高表达状态,具有促癌作用,可能是潜在的胶质瘤治疗靶点。

关键词: lncRNA, 胶质瘤, 细胞增殖, 侵袭

Abstract: Objective The aim of this study was to detect the expression of lncRNA PSMA3-AS1 in glioma tissues and cell lines(U251、LN229、T98G and A172)and its effect on glioma cells.Methods Quantitative qRT-PCR method was used to evaluate the expression of PSMA3-AS1 in 35 glioma tissues;Combined with TCGA databases,the correlation between PSMA3-AS1 expression and the degree of malignancy and the prognosis of glioma patients was analyzed;Cell proliferation and invasion were explored in U251 and T98G glioma cells;The effects of PSMA3-AS1 on invasion related proteins MMP-2/9 in glioma cells were detected by Western blot assay.Results TCGA database indicated that PSMA3-AS1 was increased in glioma tissues,and the expression of PSMA3-AS1 in 35 paired glioma tissues and glioma cells was significantly increased than in adjacent normal brain tissues(P＜0.05).The expression of PSMA3-AS1 was correlated with tumor mean diameter(P=0.007),and high PSMA3-AS1 exhibited poorer overall survival in glioma patients(P=0.042).After knockdown the level of PSMA3-AS1,the proliferation and invasion abilities of glioma cells were significantly inhibited and the expression of MMP-2/MMP9 were decreased in glioma cells(P＜0.05).Conclusion The level of PSMA3-AS1 is significantly higher in glioma tissues with a role of promoting cancer in glioma and may be a potential therapeutic target.

Key words: lncRNA, Glioma, Cell proliferation, Invasion

中图分类号:

R739.41

张遥, 范丹. 长链非编码RNA PSMA3-AS1在人胶质瘤细胞中的表达及作用研究[J]. 实用肿瘤学杂志, 2020, 34(3): 214-219.

ZHANG Yao,FAN Dan. The expression and role of LncRNA PSMA3-AS1 in human glioma cells[J]. Journal of Practical Oncology, 2020, 34(3): 214-219.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2020.03.005

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I3/214

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