PD-L1与MTA1在口腔鳞癌中的表达与临床意义

doi:10.11904/j.issn.1002-3070.2020.03.006

摘要/Abstract

摘要： 目的探讨程序性死亡配体1(Programmed death-Ligand1,PD-L1)与转移相关基因1(Metastasis associated gene1,MTA1)在口腔鳞癌组织以及正常口腔粘膜组织中的表达情况、分布状态,二者的相关性及临床意义。方法应用免疫组化方法检测PD-L1与MTA1在45例口腔鳞癌组织及20例正常口腔粘膜组织中的表达情况,分析二者表达的相关性及二者与口腔鳞癌组织临床病理学参数的关系。结果 PD-L1在口腔鳞癌组织及正常口腔粘膜组织中的阳性表达率分别为75.56%和15.00%,MTA1在口腔鳞癌组织及正常口腔粘膜组织中的阳性表达率分别为66.67%和10.00%,口腔鳞癌组织及正常粘膜组织的相对表达量具有统计学意义差异(P＜0.05)。PD-L1与MTA1的表达与口腔鳞癌的TNM分期有关(P＜0.05),口腔鳞癌组织中PD-L1与MTA1的表达有相关性(φ=0.366,P=0.037)。结论 PD-L1与MTA1可能在口腔鳞癌发生发展起到重要作用,可能成为潜在的新型联合靶点。

关键词: 口腔鳞癌, PD-L1, MTA1, 免疫组化

Abstract: Objective The aims of this study were to investigate the expressions of programmed death-ligand1(PD-L1)and metastasis associated gene 1(MTA1)in oral squamous cell carcinoma and normal oral mucosa tissues as well as their correlation and clinical significance of clinical pathology parameters.Methods The immunohistochemical method was used to detect the expression of PD-L1 and MTA1 in 45 cases of oral squamous cell carcinoma tissues and 20 cases of normal oral mucosa tissues,and to analyze the correlation between their clinical pathological parameters and the expression of PD-L1 and MTA1.Results The positive rates of PD-L1 in oral squamous cell carcinoma and normal oral mucosa tissues were 75.56% and 15%,respectively.The positive rates of MTA1 in oral squamous cell carcinoma and normal oral mucosa tissues were 66.67% and 10%,respectively.The differences were statistically significant(P＜0.05).The expression of PD-L1 and MTA1 was related to the TNM stage of oral squamous cell carcinoma(P＜0.05),and the expression of PD-L1 and MTA1 in oral squamous cell carcinoma tissues was correlated(r=0.34,P＜0.01).Conclusion PD-L1 and MTA1 may play an important role in the development of oral squamous cell carcinoma,and may become a potential new joint target.

Key words: Oral squamous cell carcinoma, PD-L1, MTA1, Immunohistochemistry

中图分类号:

R739.8

张连齐, 李吉辰, 朴松林, 赛音乌力吉, 元冬梅, 沈禹辰. PD-L1与MTA1在口腔鳞癌中的表达与临床意义[J]. 实用肿瘤学杂志, 2020, 34(3): 220-224.

ZHANG Lianqi,LI Jichen,PIAO Songlin,SAI Yin wuliji,YUAN Dongmei,SHEN Yuchen. Expression and clinical significance of PD-L1 and MTA1 in the oral squamous cell carcinoma[J]. Journal of Practical Oncology, 2020, 34(3): 220-224.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2020.03.006

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I3/220

参考文献

1 Valdez JA,Brennan MT.Impact of oral cancer on quality of life[J].Dent Clin North Am,2018,62:143-154.
2 Zini A,Czerninski R,Sgan-Cohen HD.Oral cancer over four decades:epidemiology,trends,histology,and survival by anatomical sites[J].Oral Pathol Med,2010,39(4):299-305.
3 Osipov A,Saung MT,Zheng L.Small molecule immunomodulation:the tumor microenvironment and overcoming immune escape[J].Immunother Cancer,2019,7(1):224.
4 Sun H,Zhang M,Zhao S.Expression of PD-L1 and prognosis in breast cancer:a meta-analysis[J].Oncotarget,2017,8(19):31347-31354.
5 Fusi A,Festino L,Botti G.PD-L1 expression as a potential predictive biomarker[J].Lancet Oncol,2015,16(13):1285-1287.
6 Taube JM,Klein A,Brahmer JR.Association of PD-1,PD-1 ligands,and other features of the tumor immune micro environment with response to anti-PD-1 therapy[J].Clin Cancer Res,2014,20(19):5064-5074.
7 Zou W,Chen L.Inhibitory B7-family molecules in the tumour microenvironment[J].Nat Rev Immunol,2008,8(6):467-477.
8 Kim JM,Chen DS.Immune escape to PD-L1/PD-1 blockade:seven steps to success(or failure)[J].Ann Oncol,2016,27(8):1492-1504.
9 Li DQ,Pakala SB,Nair SS.Metastasis-associated protein 1/nucleosome remodeling and histone deacetylase complex in cancer[J].Cancer Res,2012,72(2):387-394.
10 He X,Zhou C,Zheng L.Overexpression of MTA1 promotes invasiveness and metastasis of ovarian cancer cells[J].Med Sci,2014,183(3):433-438.
11 Yu Y,Zhao J,Li X.Knockdown of MTA1 inhibits the proliferation and apoptosis of esophageal cancer cells[J].Zhonghua Zhong Liu Za Zhi,2020,42(3):197-202.
12 Xue H,Wang H,Liu J.MTA1 downregulation inhibits malignant potential in a small cell lung cancer cell line[J].Oncol Rep,2015,33(2):885-892.
13 Nagata M,Wada K,Nakajima A.Role of myeloid cell leukemia-1 in cell growth of squamous cell carcinoma[J].Pharmacol Sci,2009,110(3):344-353.
14 王永霞,裴华,刘珊.MTA1基因表达在口腔鳞癌发生、发展中作用的研究[J].重庆医学,2012,41(28):2905-2906.
15 Romero Y,Wise R,Zolkiewska A.Proteolytic processing of PD-L1 by ADAM proteases in breast cancer cells[J].Cancer Immunol Immunother,2020,69(1):43-55.
16 Shan T,Chen S,Wu T.PD-L1 expression in colon cancer and its relationship with clinical prognosis[J].Int J Clin Exp Pathol,2019,12(5):1764-1769.
17 Huang W,Ran R,Shao B.Prognostic and clinicopathological value of PD-L1 expression in primary breast cancer:a meta-analysis[J].Breast Cancer Res Treat,2019,178(1):17-33.
18 张群,李多杰.PD-1/PD-L1在食管鳞癌中的研究进展[J].实用肿瘤学杂志,2017,31(3):250-253.
19 Bellmunt J,Powles T,Vogelzang NJ.A review on the evolution of PD-1/PD-L1 immunotherapy for bladder cancer:the future is now[J].Cancer Treat Rev,2017,54:58-67.
20 Fei Z,Deng Z,Zhou L.PD-L1 induces epithelial mesenchymal transition in nasopharyngeal carcinoma cells through activation PI3K/AKT pathway[J].Oncol Res,2019,27(7):801-807.
21 Kaur E,Gupta S,Dutt S.Clinical implications of MTA proteins in human cancer[J].Cancer Metastasis Rev,2014,33(4):1017-1024.
22 Sun X,Zhang Y,Li B.MTA1 promotes the invasion and migration of pancreatic cancer cells potentially through the HIF-α/VEGF pathway[J].J Recept Signal Transduct Res,2018,38(4):352-358.
23 Wang J,Qian H,Wang H.Effects of MTA1 on biological behaviors of gastric cancer cells[J].Zhonghua Zhong Liu Za Zhi,2018,40(8):580-586.
24 Mao X,Chen H,Wang H.MTA1 expression correlates significantly with ER-alpha methylation in breast cancer[J].Tumour Biol,2012,33(5):1565-1572.
25 Liu Y,Chen D,Qiu X.Relationship between MTA1 and spread through air space and their joint influence on prognosis of patients with stage Ⅰ~Ⅲ lung adenocarcinoma[J].Lung Cancer,2018,124:211-218.
26 De Craene B,Berx G.Regulatory networks defining EMT during cancer initiation and progression[J].Nature Reviews Cancer,2013,13:97-110.
27 Tuncay Cagatay S,Cimen I,Savas B.MTA-1 expression is associated with metastasis and epithelial to mesenchymal transition in colorectal cancer cells[J].Tumour Biol,2013,34(2):1189-1204.
28 Hamatsu T,Rikimaru T,Yamashita Y.The role of MTA1 gene expression in human hepatocellular carcinoma[J].Oncol Rep,2003,10(3):599-604.
29 Ma K,Fan Y,Dong X.MTA1 promotes epithelial to mesenchymal transition and metastasis in non-small-cell lung cancer[J].Oncotarget,2017,8(24):38825-38840.
30 Zou B,Xu K,Liu X.A meta-analysis of the relationship between the expression of PD-L1 and the prognosis and clinicopathological features of OSCC[J].Shanghai Stomatology,2018,27(1):100-105.
31 Tojyo I,Shintani Y,Nakanishi T.PD-L1 expression correlated with p53 expression in oral squamous cell carcinoma[J].Maxillofac Plast Reconstr Surg,2019,41(1):56.
32 Zhao R,Song Y,Wang Y.PD-1/PD-L1 blockade rescue exhausted CD8⁺T cells in gastrointestinal stromal tumours via the PI3K/Akt/mTOR signalling pathway[J].Cell Prolif,2019,52(3):e12571.
33 Gao Y,Yang J,Cai Y.IFN-γ-mediated inhibition of lung cancer correlates with PD-L1 expression and is regulated by PI3K-AKT signaling[J].Int J Cancer,2018,143(4):931-943.

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