Abstract: Objective The aim of this study was to construct the stable cell line of colorectal cancer HT29 cells with high expression of DAB2IP and its effect on invasion and migration of HT29 cells.Methods The pcDNA3.1 plasmid carrying the DAB2IP gene sequences was transfected into HT29 cells.After 24 hours,10μg/mL of G418 was added the cells to screen the cells with high expression of DAB2IP.Two weeks later,DAB2IP at levels of mRNA and protein in screened cells was detected to verify high expression of DAB2IP by real-time quantitative PCR(RT-qPCR)and Western blot.Further,the effects of DAB2IP on the invasion and migration of HT29 cells were detected by Trans well and scratch assays.Finally,the expression of E-cadherin and vimentin proteins was detected to explore the mechanism of DAB2IP affecting the invasion and migration ability of HT29 cells by Western blot.Results After screening with G418,the mRNA and protein levels of DAB2IP were significantly higher than those of the control cells.Over-expression of DAB2IP could significantly inhibit the invasion and migration of HT29 cells;the difference was statistically significant(P＜0.001).Over-expression of DAB2IP could promote the expression of E-cadherin protein and inhibit the expression of vimentin protein in TH29 cells.Conclusion The HT29 stable cell line with high expression of DAB2IP was successfully screened.Over-expression of DAB2IP can inhibit the invasion and migration in HT29 cells by inhibiting the process of cell epithelial-mesenchymal transition(EMT).

Key words: DAB2IP, HT29 cells, Invasion and migration, EMT