Abstract: Objective To investigate the expressions of HMGA2 and Twist in gastric adenocarcinoma and their association of pithelial-mesenchymal transformation(EMT)-related makers of E-cadherin and Vimentin.Methods Expressions of HMGA2,Twist、E-cadherin and Vimentin in 80 cases of gastric adenocarcinoma and in 20 cases of normal gastric tissues were assayed by immunohistochemical staining(IHC).We also compared with clinicopathological data.Results HMGA2,Twist、E-cadherin and Vimentin positive expression rates were 65%,68.75%,26.25% and 67.5%,respectirely,in gastric adenocarcinoma.The expression of HMGA2 was positively correlated with that of Twist(P＜0.05).The expressions of HMGA2 and Twist were significantly correlated with lymph node metastasis and TNM stage(P＜0.05),the low expression of E-cadherin(P＜0.05)and the increased expression of Vimentin(P＜0.05).Conclusion HMGA2 and Twist over-expressed in gastric adenocarcinoma,and related to EMT.It promotes epithelial-mesenchymal transitions phenotype,and then induces invasion and metastasis of gastric adenocarcinoma.