Abstract:

Objective To investigate the role of HMGA2 expression and epithelial to mesenchymal cell transformation in the regulation of TGF-β1 in human gastric cancer cells.Methods Human gastric cancer cell SGC-7901 was treated by TGF-β1 at different time points(0,3,6,24,48,72h).We observed the changes in cell morphology of SGC-7901 through the microscope;HMGA2 assay,E-cadherin,Vimentin protein expression were performed by Western blot;cell invasion capability was analyzed by transwell chamber assay.Results (1)With the prolonged duration of action of TGF-β1,SGC-7901 changes in cell morphology,cells gradually became longer and larger.(2)In the role of TGF-β1,gastric cancer cells SGC-7901 are varying degrees of HMGA2 expression and E-cadherin、Vimentin.HMGA2 protein begun to express from 0h to 6hrs.and HMGA2 protein became the strongest at 6hrs and decreased slightly from 24hrs.E-cad protein expression at 0h begun at 3hrs highest expression,and the reduction started from 3hrs.Vimentin protein was the highest at 48hrs then gradually decreased.Conclusion In the TGF-β1 induced the process of epithelial to mesenchymal cell transformation in gastric cancer cells SGC-7901,HMGA2 protein play an important role.HMGA2 can be used as snail promoter,combined with Smads,promotes the process of epithelial-mesenchymal transformation.HMGA2 may also be regulated by Wnt / β-Catenin signaling pathway on growth and apoptosis of tumor cells.

Key words: SGC-7901, EMT, HMGA2