Abstract: Objective In this paper,next-generation sequencing(NGS)technology was used to analyze the genetic mutation characteristics of ascending and descending nasopharyngeal carcinoma,to find the differences between them,and to explore the relationship between high-frequency mutation genes and nasopharyngeal carcinoma(NPC)invasion and regional lymph node metastasis.Methods Forty cases of NPC patients diagnosed(20 cases ascending and descending NPC a group)were collected in Guizhou Provincial People's Hospital from January 2014 to December 2018.DNA extraction was performed using Illumina.The sequencing platform captured and sequenced 400 tumor-related genes for detection mutations.The sequencing results were combined with relevant clinical data for analysis.Results In 40 specimens of NPC histopathological tissues,ABCA13,CACNA1F,CELSR2,CEP170B genes were detected to be mutated in descending NPC;ABCC2,ALPK2,ASB15,ASRGL1 and other genes have higher mutations in ascending NPC than descending NPC.Among them,centrosome protein 170B(Centrosomal protein 170B,CEP170B)has the highest mutation rate in descending NPC,with a mutation rate of 57%.There was no statistically significant difference in tumor mutation burden and tumor heterogeneity between G1 and G2 groups.Conclusion The CEP170B gene has a high frequency of mutations in the descending NPC group.Because descending NPC is prone to extensive lymph node metastasis,the results suggest that this gene may be closely related to the local regional lymph node metastasis of NPC,and may become a clinical NPC Potential targets for treatment.

Key words: Nasopharyngeal carcinoma, Next-generation sequencing, CEP170B, Gene mutation