Abstract: Objective The aims of this study were to investigate the role of miRNA-126 in the occurrence of gastric cancer cells by detecting miRNA-126 and predicting the expression of target genes K-Ras and MMP-7 in gastric cancer tissues. Which may provide a new theoretical basis for the study of miRNA-126/K-Ras and MMP-7 pathway.Methods A total of 50 gastric cancer tissues and normal tissues of gastric cancer patients who were diagnosed and accepted treatment in the Affiliated Hospital of Inner Mongolia Medical University from March,2015 to December,2015 were collected.Immunohistochemical staining was used to detect the expression of K-Ras and MMP-7 in gastric cancer tissues and normal tissues.Real-time PCR was used to detect the expression of miRNA-126 in gastric cancer tissues and normal tissues.Results K-Ras and MMP-7 were highly expressed in gastric cancer tissues.miRNA-126 was lowly expressed in gastric cancer tissues.K-Ras and MMP-7 was positively correlated in gastric cancer tissues.miRNA-126 was lowly expressed when K-Ras and MMP-7 expression was positive.Conclusion K-Ras and MMP-7 are carcinogenic factors of gastric cancer,and miRNA-126 is a tumor suppressor of gastric cancer,which is a antagonistic factor of K-Ras and MMP-7.K-ras and MMP-7 are the target genes of miRNA-126,and miRNA-126 exists to inhibit the interaction of K-Ras and MMP-7.The miRNA-126/K-Ras,MMP-7 pathway is expected to became a new direction of gastric cancer research and treatment.

Key words: Gastric cancer, miRNA-126, K-Ras, MMP-7