Abstract: Objective To investigate the correlation of the telomerase activity and radiosensitivity of surviving progeny from different doses irradiated cervical carcinoma cell.Methods The HeLa cells were irradited with 0, 2, 6, 10Gy of X-ray for three times and the surviving progeny were subculture.There were four groups:HeLa, HeLa-2, HeLa-6, HeLa-10.The telomerase activity was measured by the polymerase chain reaction-based telomeic repeat amplification protocol coupled with ELISA.And the expression of the hTERT mRNA were detected by the RT-PCR assay.The radiosesitivity index was examined by clone formation assay.Results The HeLa cells were treated by different doses of irradiation.The telomerase activity of HeLa group HeLa-2 group HeLa-6 group was(1.54±0.01), (1.87±0.01), (2.03±0.02)and the expression of hTERT mRNA was increased with the irradiation dose and was(0.44±0.03), (0.53±0.04), (0.74±0.07).Each parameter had significant differences among the survival progenies(P＜0.05).But the HeLa-10 group was(1.02±0.024)and(0.21±0.02)significantly reduced.Each group′s radiosensitivity was declined with the different irradiation dose.The telomerase activity and the expression of the hTERT mRNA was negatively correlated with radiosensitivity of each groups(r=-0.927, P＜0.01:r=-0.904, P＜0.01).Conclusion Radiosensitivity of the surviving progeny correlates with the telomerase activity and the expression of hTERT.