Abstract: Objective The purpose of this study is to investigate the expression of human epidermal growth factor receptor-2(HER-2)in gastric carcinoma tissue and normal gastric tissue of these patients,and its relationship with the clinical pathological characteristic,the microvessel density(MVD)and early post-operative recurrence,and to analyze the clinical significance of expression of HER-2 in gastric carcinoma,in order to lay the theoretical foundation of effective therapy for gastric carcinoma.Methods The clinical data of 398 cases of gastric cancer and 363 cases of their adjacent non tumorous gastric tissue of Liaoning Cancer Hospital from March 2012 to July 2013 were analyzed retrospectively.The expression of HER-2 gene was detected by IHC method and Fish method.The expressive rate of HER-2 in gastric carcinoma tissue and normal gastric tissue of these patients were compared,and its relationship with the clinical pathological characteristic,MVD and early post operative recurrence were also analyzed.Results (1)Expressive rate of HER-2 in 398 cases of gastric cancer tissue was 14.07%(56/398),which was higher than that in all the adjacent non tumorous gastric tissue presented none expression 0(0/363)(P＜0.05);(2)In gastric cancer tissue of 398,the expression of HER-2 was related with Lauren type,tumor site,vessel invasion status,TNM stage,lymph node metastases(P＜0.05),and it had no correlation with age,sex,tumor size,histological differentiation degree,growth patterns of cancer(P＞0.05).Expressive rate of HER-2 in T4 stage was higher than(T₁+T₂+T₃)stage,but the difference was not statistically significant(P＞0.05).(3)The value of MVD in HER-2 expressive group(58.63±19.97)was significantly higher than those in HER-2 non expressive group(49.04±19.25).A positive significant correlation was found between HER-2 and MVD expression using the rank correlation matrix(P＜0.001,r=4.33).(4)1 year and 1.5 year of early post operative recurrence rate in HER-2 expressive group were 16.00%、38.00%,in HER-2 non expressive group were 8.81%,25.53%,the difference was not statistically significant(P＞0.05).Conclusion There is excessive expression of HER-2 in gastric cancer tissue,excessive expression of HER-2 patients with gastric cancer is correlated with Lauren type,tumor location,vessel invasion status,TNM stage,lymph node metastases;it displays no correlation with age,sex,tumor size,histological differentiation degree,growth patterns of cancer.HER-2 expression is positively associated with MVD value.Excessive expression of HER-2 is possible to be correlative with early post operative recurrence,and it must have further follow up to confirm.

Key words: Gastric cancer, Immunohistochemical, Human epidermal growth factor receptor-2, Microvessel density, Neoplasm recurrence rate